Mouse anti-Rat CEACAM1 Monoclonal Antibody | anti-CEACAM1 antibody

Anti-CEACAM1 Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8112838

• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CEACAM1; Monoclonal Antibody; Anti-CEACAM1 Antibody (APC); Mouse Monoclonal; CEACAM?1/CD66a Antibody (APC); Mouse MAb; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); anti-CEACAM1 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 03
• Specificity: Rat CEACAM?1/CD66a
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 2ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CEACAM1 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Rat CEACAM-1/CD66a Protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CEACAM?1/CD66a (rR CEACAM?1/CD66a) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Rat CEACAM1(CD66a) expression on SD rat splenocytes. Cells were stained with APC-conjugated anti-Rat CEACAM1(CD66a). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-CEACAM1 antibody

The carcinoembryonic-antigen-related cell-adhesion molecule (CEACAM) family of proteins has been implicated in various intercellular-adhesion and intracellular-signalling-mediated effects that govern the growth and differentiation of normal and cancerous cells. CEACAM1, also known as biliary glycoprotein I (BGP I) and CD66a, is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. The highly glycosylated CEACAM1 contains one N-terminal V-type Ig-like domain and three C2-type Ig-like domains within its ECD, and one ITIM motif and a calmodulin binding site in the cytoplasmic region. CEACAM1 is a surface glycoprotein expressed on various blood cells, epithelial cells, and vascular cells. It was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Product Categories/Family for anti-CEACAM1 antibody

Cancer Drug Targets ITIM/ITAM Immunoreceptors and Related Molecules

References

Gray-Owen SD, et al. (2006) CEACAM1: contact-dependent control of immunity. Nat Rev Immunol. 6(6): 433-46.Gu A, et al. (2009) Role of Ceacam1 in VEGF induced vasculogenesis of murine embryonic stem cell-derived embryoid bodies in 3D culture. Exp Cell Res. 315(10): 1668-82.Wong C, et al. (2009) CEACAM1 negatively regulates platelet-collagen interactions and thrombus growth in vitro and in vivo. Blood. 113(8): 1818-28.