Rabbit anti-Human LAG-3 Polyclonal Antibody | anti-LAG-3 antibody

LAG-3 (Lymphocyte Activation Gene 3 Protein, LAG3, CD223, FDC, Protein FDC) (FITC)

Cat. Num. AAA6484638

• Gene Names: LAG3; CD223
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LAG-3; Polyclonal Antibody; LAG-3 (Lymphocyte Activation Gene 3 Protein; LAG3; CD223; FDC; Protein FDC) (FITC); Lymphocyte-activation Gene 3; anti-LAG-3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LAG3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LAG-3 antibody: Western Blot (WB), FLISA, Immunohistochemistry (IHC)
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 103-132 in the central region of human LAG3, UniProt Accession #P18627.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS623650 (Center) in K562 cell line lysates (35ug/lane). LAG3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of MBS623650 in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-LAG-3 antibody

Antibodies; CD Markers

References

1. Smyth, D.J., et.al., BMC Med. Genet. 7, 20 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 167614500
• NCBI GeneID: 3902
• NCBI Accession #: NP_002277.4
• NCBI GenBank Nucleotide #: NM_002286.5
• UniProt Accession #: P18627; Q7Z643; A8K7T9
• Molecular Weight: 39,103 Da
• NCBI Official Full Name: lymphocyte activation gene 3 protein
• NCBI Official Synonym Full Names: lymphocyte-activation gene 3
• NCBI Official Symbol: LAG3
• NCBI Official Synonym Symbols: CD223
• NCBI Protein Information: lymphocyte activation gene 3 protein
• UniProt Protein Name: Lymphocyte activation gene 3 protein
• UniProt Gene Name: LAG3
• UniProt Synonym Gene Names: FDC; LAG-3
• UniProt Entry Name: LAG3_HUMAN

Uniprot Description

LAG3: Involved in lymphocyte activation. Binds to HLA class-II antigens.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.32

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: transmembrane receptor activity; antigen binding; MHC class II protein binding

Biological Process: cell surface receptor linked signal transduction; negative regulation of interleukin-2 biosynthetic process; positive regulation of natural killer cell mediated cytotoxicity; negative regulation of T cell activation