Mouse anti-Human, Rat CD34 Monoclonal Antibody | anti-CD34 antibody

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Cat. Num. AAA4381428

• Reactivity: Human, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: CD34; Monoclonal Antibody; CD34 (Hematopoietic Stem Cell & Endothelial Marker); Hematopoietic Progenitor Cell Antigen; HPCA1; Mucosialin; anti-CD34 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ICO-115
• Specificity: This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 328

• Applicable Applications for anti-CD34 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry: 5ul per test per one million cells (or 5ul per 100ul of whole blood); IF: 1:50-1:100
• Positive Control: KG-1 cells. Human tonsil or angiosarcoma.
• Immunogen: Blast cells of a chronic myeloid leukemia patient
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 1q32.2
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow cytometry of KG-1 cells using CD34 Monoclonal Antibody (ICO-115) (red) and isotype control Ab (green).)

References

1. Kishimoto et al., Leucocyte typing Gariand Publishing. 1997. 2. Baryshnikov A, and Tonevitsky A, Monoclonal antibodies in laboratory and clinic. Thesis p212, 1997. 3. Frolova, E., Baryshnikov A.Y., Frenkel M. et al., Monoclonal antibody ICO-115 to human CD34 antigen. Herald of the Cancer Research Center of RAMS. Suppl, p. 10-12, 1994. 4. Sato T et al. Blood 94:2548-2554 (1999). 5. Felshow DM et al. Blood 97:3768-3775 (2001).

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• UniProt Accession #: P28906
• Molecular Weight: 90-110kDa
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34
• UniProt Entry Name: CD34_HUMAN

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: intercellular bridge; lysosome; integral to plasma membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; basal plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: sulfate binding; carbohydrate binding; transcription factor binding

Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration