Western Blot (WB)
(WB: Rat brain total membrane proteins preparation was probed with MBS615828. Lane 1: MBS615828 at 1:200. Lane 2: MBS615828 preincubated with MBS615828A, control peptide.)
Rabbit anti-Rat Kv4.2 Polyclonal Antibody | anti-kv4.2 antibody
Kv4.2 (Shal1, RK5, Kcnd2, Voltage-gated Potassium Channel)
Reactivity
Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Kv4.2; Polyclonal Antibody; Kv4.2 (Shal1; RK5; Kcnd2; Voltage-gated Potassium Channel); Anti -Kv4.2 (Shal1; anti-kv4.2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat Kv4.2. Epitope Location: Intracellular, C-terminal part. The epitope specific for this antibody is not present in any other known proteins.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.025% sodium azide. Reconstitute with sterile ddH2O.
Applicable Applications for anti-kv4.2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation.
Dilution: Western Blot: 1:200; Rat brain membranes using ECL.
Immunohistochemistry: Rat brain sections.
Note: Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilution determined by the researcher.
Dilution: Western Blot: 1:200; Rat brain membranes using ECL.
Immunohistochemistry: Rat brain sections.
Note: Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal dilution determined by the researcher.
Immunogen
Synthetic peptide corresponding to aa454-469, (C)SNQLQSSEDEPAFVSK of rat Kv4.2 (Accession Q63881). Species Sequence Homology: mouse and human: 94%
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB: Rat brain total membrane proteins preparation was probed with MBS615828. Lane 1: MBS615828 at 1:200. Lane 2: MBS615828 preincubated with MBS615828A, control peptide.)
Western Blot (WB)
(WB: Rat brain total membrane proteins preparation was probed with MBS615828. Lane 1: MBS615828 at 1:200. Lane 2: MBS615828 preincubated with MBS615828A, control peptide.)
Related Product Information for anti-kv4.2 antibody
Kv4.2, a voltage-gated K+ (Kv) channel subunit, has been suggested to be the key component of the subthreshold A-type K+ currents (I(SA)s) recorded from the specific subcellular compartments of certain CNS neurons. A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart. Kv4.2 is a Shal-type potassium channel that passes an A-type current and is localized to dendrites and cell bodies in the hippocampus. Deletion of the Kv4.2 gene and a loss of Kv4.2 protein resulted in a specific and near-complete elimination of A-type K+ currents from the apical dendrites of CA1 pyramidal neurons. The absence of dendritic Kv4.2-encoded A-type K+ currents led to an increase of bAP amplitude and an increase of concurrent Ca2+ influx.
Product Categories/Family for anti-kv4.2 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Kv4.2 kv4.2 (Catalog #AAA615828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kv4.2 (Shal1, RK5, Kcnd2, Voltage-gated Potassium Channel) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kv4.2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Dilution: Western Blot: 1:200; Rat brain membranes using ECL. Immunohistochemistry: Rat brain sections. Note: Dilutions should be made using a carrier protein such as BSA (1-3%). Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the Kv4.2 kv4.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kv4.2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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