Rabbit anti-Human ABL1 Polyclonal Antibody | anti-ABL1 antibody

ABL1 (Tyrosine-protein kinase ABL1, ABLJTK7, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine-protein kinase 1, Proto-oncogene c-Abl) (AP)

Cat. Num. AAA6460904

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ABL1; Polyclonal Antibody; ABL1 (Tyrosine-protein kinase ABL1; ABLJTK7; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl) (AP); anti-ABL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ABL1. Has not been tested for cross-reactivity with ABL2.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 1182

• Applicable Applications for anti-ABL1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1,000; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within the amino acid range 220-249 of human ABL1, UniProt Accession #P00519.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

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Product Categories/Family for anti-ABL1 antibody

Antibodies; Tyrosine Kinases

References

1. Cao, C., et al., Biochemistry 42(35):10348-10353 (2003). 2. Cao, C., et al., J. Biol. Chem. 278(32):29667-29675 (2003). 3. Kruh, G.D., et al., Proc. Natl. Acad. Sci. USA 87(15):5802-5806 (1990). 4. Kruh, G.D., et al., Science 234(4783):1545-1548 (1986). 5. Bianchi, C., et al., FEBS Lett. 527 (1-3), 216-222 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 6382062
• NCBI GeneID: 27
• NCBI Accession #: NP_009298
• NCBI GenBank Nucleotide #: NP_009298.1
• UniProt Accession #: P42684
• Molecular Weight: 128.343
• NCBI Official Full Name: tyrosine-protein kinase ABL2 isoform b
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: tyrosine-protein kinase ABL2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Oncoprotein; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; actin cytoskeleton; phagocytic cup

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; positive regulation of protein binding; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of apoptosis; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; regulation of endocytosis; visual learning; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; actin filament bundle formation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; protein modification process; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; negative regulation of Rho protein signal transduction; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuron remodeling