Rabbit Kv2.1Potassium Channel Polyclonal Antibody | anti-Kcnb1 antibody

Kv2.1Potassium Channel

Cat. Num. AAA395978

• Gene Names: Kcnb1; Shab; Kv2.1; DRK1PC; Kcr1-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: Kv2.1Potassium Channel; Polyclonal Antibody; anti-Kcnb1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 853

• Applicable Applications for anti-Kcnb1 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide derived from the rat Kv2.1 potassium channel conjugated to KLH
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-Kcnb1 antibody

The Kv2.1 potassium channel is a voltage-gated channel protein which belongs to the delayed rectifier class and to the Shah potassium channel subfamily. Potassium channels are mainly found in plasma membranes but are not generally distributed over the cell surface. Potassium channels catalyze the rapid permeation of potassium ions while rejecting biologically abundant potential competitors such as sodium, calcium and magnesium. Ion selectivity and high through put rate of potassium channels is accomplished by precise co-ordination of dehydrated potassium by the protein and multiple ion occupancy within the permeation pathway. All potassium channels carry out the formation of a transmembrane leak specific for potassium ions. Since cells almost universally maintain cytoplasmic potassium concentrations higher than those extracellularly, the opening of a potassium channel implies a negative ongoing change in electrical voltage across the cell membrane. This may result in termination of the action potential of electrically excitable cells including nerve, muscle and pancreatic beta cells. In non?excitable cells, potassium channels play important roles in the cellular potassium recycling required for electrolyte balance effected by the renal epithelium.

Product Categories/Family for anti-Kcnb1 antibody

Polyclonal Antibody; Ion Channels

NCBI and Uniprot Product Information

• NCBI GI #: 6981120
• NCBI GeneID: 25736
• NCBI Accession #: NP_037318.1
• NCBI GenBank Nucleotide #: NM_013186.1
• UniProt Accession #: P15387
• Molecular Weight: 95,637 Da
• NCBI Official Full Name: potassium voltage-gated channel subfamily B member 1
• NCBI Official Synonym Full Names: potassium voltage gated channel, Shab-related subfamily, member 1
• NCBI Official Symbol: Kcnb1
• NCBI Official Synonym Symbols: Shab; Kv2.1; DRK1PC; Kcr1-1
• NCBI Protein Information: potassium voltage-gated channel subfamily B member 1; DRK1; potassium channel Kv2.1; delayed rectifier potassium channel 1; voltage-gated potassium channel subunit Kv2.1
• UniProt Protein Name: Potassium voltage-gated channel subfamily B member 1
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: Kcnb1
• UniProt Synonym Gene Names: DRK1
• UniProt Entry Name: KCNB1_RAT

NCBI Description

voltage gated potassium channel; involved in the excitability in nerve and muscle [RGD, Feb 2006]

Uniprot Description

Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Channels open or close in response to the voltage difference across the membrane, letting potassium ions pass in accordance with their electrochemical gradient. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure: Heteromultimer with KCNG2, KCNG3, KCNG4, KCNS1, KCNS2, KCNS3 and KCNV2

By similarity.

Subcellular location: Membrane; Multi-pass membrane protein.

Tissue specificity: In the brain, the greatest density occurs in the cerebral cortex, followed by the hippocampus, cerebellum, and olfactory bulb. In peripheral tissues it is most prominent in retina and kidney. Also present in cardiac muscle tissue of the atrium and ventricle and in skeletal muscle.

Developmental stage: Levels remain constant throughout postnatal development. Ref.6

Domain: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

Post-translational modification: Highly phosphorylated on serine residues in the C-terminal. Differential phosphorylation on a subset of serines allows graded activity-dependent regulation of channel gating. Phosphorylation on Ser-457, Ser-541, Ser-567, Ser-607, Ser-655 and Ser-719 as well as the N-terminal Ser-15 are all regulated by calcineurin-mediated dephosphorylation. Particularly, Ser-607 and Tyr-128 are significant sites of voltage-gated regulation through phosphorylation/ dephosphorylation activities. Tyr-128 can be dephosphorylated by PTPalpha and cyt-PTPepsilon. Phosphorylation levels on Ser-607 are supersensitive to neuronal activity. Phosphorylation on Ser-567 is reduced during postnatal development with low levels at P2 and P5. Levels then increase to reach adult levels by P14. Phosphorylation levels on Ser-564 and Ser-607 are greatly reduced during seizures, by 40% and 85% respectively. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Miscellaneous: As phosphorylation levels on Ser-607 are highly sensitive to changes in neuronal activity, the immunoreactivity of Ser-607P could serve as a bidirectional biomarker for neuronal activity.

Sequence similarities: Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Kv2.1/KCNB1 sub-subfamily. [View classification]

Sequence caution: The sequence CAA34497.1 differs from that shown. Reason: Erroneous initiation.