Rabbit anti-Human SENP1 Polyclonal Antibody | anti-SENP1 antibody

SENP1 (Sentrin, SUMO-specific protease 1)

Cat. Num. AAA612189

• Gene Names: SENP1; SuPr-2
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: SENP1; Polyclonal Antibody; SENP1 (Sentrin; SUMO-specific protease 1); Anti -SENP1 (Sentrin; anti-SENP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Reacts with the human SENP1 (~73kD). Reactivity has been confirmed with DYKDDDK-HA-tagged-SENP1 transfected Hep2 cell lysates and human HL-60 acute myeloid leukemia cell lysates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-SENP1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1-3ug/ml; Immunoprecipitation: 7ug/ml
• Immunogen: Synthetic peptide derived from the mid region of the human SENP1 protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis SENP 1 in whole HL60 cell lysate. SENP1 (Arrow) was detected using anti-SENP1(Lane B) but not pre-immune serum (lane A). Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Related Product Information for anti-SENP1 antibody

The mammalian SUMO-specific protease (SENP1) is active against sentrin, but not ubiquitin or NEDD8-modified proteins in vivo (1). SENP1 provides a unique tool to study the role of sentrinization in the biological function of Pml and in the pathogenesis of acute promyelocytic leukemia (1). SENP1 localizes to the nucleus mainly in discrete subdomains, a subset of which co-localized with Pml nuclear bodies (NBs) (2). Both The herpes simplex virus 1 (HSV-1) immediate early regulatory protein (ICP0) and SENP1 protease promote the loss of SUMO-1 from the nucleus (2). SENP1 recruitment may be an important (though not sufficient) role in the modulation of SUMO-1 and NBs by ICP0 (2). SENP1 itself may be a target for SUMO-1 modification occurring at a nonconsensus site. SENP1 localization is influenced by expression and localization of SUMO-1-conjugated target proteins within the cell (3).

Product Categories/Family for anti-SENP1 antibody

Antibodies; Abs to Ubiquitin

NCBI and Uniprot Product Information

• NCBI GI #: 7657550
• NCBI GeneID: 29843
• NCBI Accession #: NP_055369.1
• NCBI GenBank Nucleotide #: NM_014554.2
• UniProt Accession #: Q6N001; Q7Z3G1; Q9P0U3
• Molecular Weight: 17,790 Da
• NCBI Official Full Name: sentrin-specific protease 1
• NCBI Official Synonym Full Names: SUMO1/sentrin specific peptidase 1
• NCBI Official Symbol: SENP1
• NCBI Official Synonym Symbols: SuPr-2
• NCBI Protein Information: sentrin-specific protease 1; SUMO1/sentrin specific protease 1; sentrin/SUMO-specific protease SENP1
• UniProt Protein Name: Putative uncharacterized protein DKFZp686L1432
• Protein Family: Sentrin-specific protease
• UniProt Gene Name: DKFZp686L1432
• UniProt Synonym Gene Names: SENP1
• UniProt Entry Name: Q6N001_HUMAN

NCBI Description

The covalent modification of proteins by the small ubiquitin (UBB; MIM 191339)-like protein SUMO (see SUMO1, MIM 601912) is implicated in the regulation of nucleocytoplasmic transport, genomic stability, gene transcription, and other processes. Sumoylation is catalyzed on target lysine residues by a multienzyme process and is reversed by desumoylating enzymes such as SENP1 (Yamaguchi et al., 2005 [PubMed 15923632]).[supplied by OMIM]

Uniprot Description

SENP1: Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity. Belongs to the peptidase C48 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 3.4.22.68; Protease

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; nucleus

Molecular Function: protein binding; endopeptidase activity; SUMO-specific protease activity

Biological Process: protein desumoylation; caspase activation; protein sumoylation; cellular protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; proteolysis; post-translational protein modification; negative regulation of proteasomal ubiquitin-dependent protein catabolic process