Rabbit anti-Human KRT8 Polyclonal Antibody | anti-KRT8 antibody

KRT8, CT (Keratin, Type II Cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, CYK8, Type-II Keratin Kb8)

Cat. Num. AAA648456

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified; Purified
• Synonyms: KRT8; Polyclonal Antibody; CT (Keratin; Type II Cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; CYK8; Type-II Keratin Kb8); Anti -KRT8; anti-KRT8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human KRT8.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20mg/ml BSA, 0.05% sodium azide.

• Applicable Applications for anti-KRT8 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1:25-1:100; Immunohistochemistry (Frozen): 1:100-1:200
• Immunogen: Synthetic peptide corresponding to human Cytokeratin 8 at C-terminal conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Liver, bile duct, Human: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Pancreas, duct, Human: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-KRT8 antibody

KRT8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation.

Product Categories/Family for anti-KRT8 antibody

Antibodies; Abs to Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 62913980
• NCBI GeneID: 3856
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: 53,704 Da
• NCBI Official Full Name: KRT8 protein, partial
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Ref.20

Subunit structure: Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Ref.15 Ref.16 Ref.19 Ref.20 Ref.21 Ref.23 Ref.27

Subcellular location: Cytoplasm. Nucleus › nucleoplasm

By similarity. Nucleus matrix

By similarity Ref.15 Ref.27.

Tissue specificity: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity. Ref.15 Ref.20

Post-translational modification: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation. Ref.13 Ref.30 Ref.35O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. Ref.13 Ref.30 Ref.35

Involvement in disease: Cirrhosis (CIRRH) [MIM:215600]: A liver disease characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Clinical features include abdomen swelling, jaundice and pulmonary hypertension.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.36

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.