Quality Control
(Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody. )
Mouse anti-Human RUVBL1 Monoclonal Antibody | anti-RUVBL1 antibody
Mouse monoclonal antibody Anti-Human RUVBL1
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody. )
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-RUVBL1 monoclonal antibody. )
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Western Blot (WB)
(Detection of human RUVBL1 by Western blot.Samples: Whole cell lysate (50 ug) from HT-1080 cells. [Lot No. 2943C1a-1]Predicted molecular weight: 50 kDa)
Western Blot (WB)
(Detection of human RUVBL1 by Western blot.Samples: Whole cell lysate (50 ug) from HT-1080 cells. [Lot No. 2943C1a-1]Predicted molecular weight: 50 kDa)
Flow Cytometry (FC/FACS)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1])
Flow Cytometry (FC/FACS)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RUVBL1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2943C1a-1])
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])
Immunocytochemistry (ICC)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RUVBL1 mAb. [Lot No. 2943C1a-1])
NCBI and Uniprot Product Information
Uniprot Description
RUVBL1: Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1- CTNNB1 complex. Belongs to the ruvB family. Forms homotypic and heterotypic interactions. Forms a multimeric complex with RUVBL2. Interacts with the transcriptional activation domain of MYC. Component of the RNA polymerase II holoenzyme complex. May also act to bridge the LEF1/TCF1-CTNNB1 complex and TBP. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. RUVBL1 interacts with EP400. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Component of the BAF53 complex, at least composed of ACTL6A/BAF53A, RUVBL1/TIP49, SMARCA2/BRM, and TRRAP/PAF400. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, IN80C, KIAA1267, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Associates with alpha and gamma tubulins, particularly during metaphase and early anaphase. Interacts with NPAT. Component of the chromatin-remodeling INO80 complex, at least composed of ACTL6A, ACTR5, ACTR8, RVBL1, RVBL2, INO80, INO80B, INO80C, INO80D and INO80E. Interacts with IGHMBP2. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 3q21
Cellular Component: nucleoplasm; Golgi apparatus; NuA4 histone acetyltransferase complex; nuclear matrix; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; nucleus
Molecular Function: DNA helicase activity; protein binding; ATPase activity; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; mitosis; nucleosome assembly; establishment and/or maintenance of chromatin architecture; DNA replication-independent nucleosome assembly at centromere; transcription, DNA-dependent; cell division; regulation of growth; spermatogenesis; DNA repair; DNA duplex unwinding; DNA recombination
Research Articles on RUVBL1
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Product Notes
The RUVBL1 ruvbl1 (Catalog #AAA120207) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human RUVBL1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RUVBL1 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the RUVBL1 ruvbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUVBL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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