Rabbit MEK1 Polyclonal Antibody | anti-MEK1 antibody

MEK1 (Phospho-Ser298) Antibody

Cat. Num. AAA9429346

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: MEK1; Polyclonal Antibody; MEK1 (Phospho-Ser298) Antibody; Dual specificity mitogen activated protein kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; Mek1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; MP2K1_HUMAN; PRKMK1; Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1); Protein kinase mitogen activated; kinase 1;; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-MEK1 (Phospho-Ser298) Antibody detects endogenous levels of MEK1 only when phosphorylated at Ser298
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/mL (varies by lot)
• Sequence Length: 393

• Applicable Applications for anti-MEK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human MEK1 around the phosphorylation site of Ser298
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Phospho-MEK1 (Ser298) in lysates of NIH-3T3, using Phospho-MEK1 (Ser298) Antibody(13194).)

Product Categories/Family for anti-MEK1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q02750
• Molecular Weight: 45
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.