Rabbit anti-Human, Mouse KNTC1 Polyclonal Antibody | anti-KNTC1 antibody

KNTC1 Antibody

Cat. Num. AAA9631220

• Gene Names: KNTC1; ROD
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KNTC1; Polyclonal Antibody; KNTC1 Antibody; HsROD; Kinetochore associated 1; ROD; Rough Deal homolog; centromere/kinetochore protein; anti-KNTC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KNTC1 Antibody detects endogenous levels of KNTC1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KNTC1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KNTC1.
• Conjugation: Unconjugated
• Subunit Structure: Interacts with ZW10; the interaction is required for stable association with the kinetochore. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH; in the complex interacts directly with ZWILCH.
• Tissue Specificity: High expression in testis.
• Function: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.
• Note: Dynamic pattern of localization during the cell cycle. At interphase, uniformly distributed throughout the cytoplasm and nucleus. By prophase and until late stages of prometaphase, a fraction of the total pool is concentrated at kinetochores. By metaphase, detected at kinetochores, along spindle fibers and most prominently at the poles. By late anaphase until the end of telophase, no longer detectable on kinetochores or along spindle fibers, but still present at the spindle poles.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KNTC1 antibody

Cytoplasm; Nucleus; Chromosome; Centromere; Kinetochore; Cytoskeleton; Spindle; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 7661960
• NCBI GeneID: 9735
• NCBI Accession #: NP_055523.1
• NCBI GenBank Nucleotide #: NM_014708.5
• UniProt Accession #: P50748; A7E2C4; B3KSG2
• Molecular Weight: 51,102 Da
• NCBI Official Full Name: kinetochore-associated protein 1
• NCBI Official Synonym Full Names: kinetochore associated 1
• NCBI Official Symbol: KNTC1
• NCBI Official Synonym Symbols: ROD
• NCBI Protein Information: kinetochore-associated protein 1
• UniProt Protein Name: Kinetochore-associated protein 1
• Protein Family: Kinetochore-associated protein
• UniProt Gene Name: KNTC1
• UniProt Synonym Gene Names: KIAA0166; HsROD; Rod; hRod

NCBI Description

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]

Uniprot Description

KNTC1: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores.

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: actin cytoskeleton; cytosol; kinetochore microtubule; nucleus; plasma membrane; spindle pole

Molecular Function: protein binding

Biological Process: cell division; mitotic cell cycle checkpoint; protein complex assembly; regulation of exit from mitosis; sister chromatid cohesion