Human Kinetochore-associated protein 1 (KNTC1) RTU ELISA Kit | KNTC1 rtu elisa kit

Human Kinetochore-associated protein 1 (KNTC1) ELISA Kit

Cat. Num. AAA4500637

• Gene Names: KNTC1; ROD
• Reactivity: Human
• Synonyms: Kinetochore-associated protein 1 (KNTC1); Human Kinetochore-associated protein 1 (KNTC1) ELISA Kit; KNTC1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 2209

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.056ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KNTC1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KNTC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KNTC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KNTC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KNTC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7661960
• NCBI GeneID: 9735
• NCBI Accession #: NP_055523.1
• NCBI GenBank Nucleotide #: NM_014708.5
• UniProt Accession #: P50748; A7E2C4; B3KSG2
• Molecular Weight: 51,102 Da
• NCBI Official Full Name: kinetochore-associated protein 1
• NCBI Official Synonym Full Names: kinetochore associated 1
• NCBI Official Symbol: KNTC1
• NCBI Official Synonym Symbols: ROD
• NCBI Protein Information: kinetochore-associated protein 1
• UniProt Protein Name: Kinetochore-associated protein 1
• Protein Family: Kinetochore-associated protein
• UniProt Gene Name: KNTC1
• UniProt Synonym Gene Names: KIAA0166; HsROD; Rod; hRod

NCBI Description

This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. Experimental evidence indicated that the encoded protein functioned in a similar manner to that of the Drosophila rough deal protein. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores (PubMed:11146660, PubMed:11590237, PubMed:15824131). Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex.