Rabbit KLK8 Polyclonal Antibody | anti-KLK8 antibody

KLK8 Polyclonal Antibody

Cat. Num. AAA3015463

• Gene Names: Klk8; NP; BSP1; Nrpn; Prss19
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: KLK8; Polyclonal Antibody; KLK8 Polyclonal Antibody; Kallikrein-8; Ovasin; Serine protease TADG 14; Tumor associated differentially expressed gene 14 protein; Brain serine protease 1; BSP1; hK8; HNP; HPN; Kallikrein 8 (neuropsin/ovasin); Kallikrein related peptidase 8; Kallikrein8; KLK 8; KLK-8; KLK8 protein type 1; KLK8 protein type 2; Neuropsin; neuropsin type 1; Neuropsin type 1; included; Neuropsin type 2; mouse; homolog of; NP; NRPN; Protease serine 19; PRSS 19; PRSS19; Serine protease 19; Serine protease kallikrein; Serine protease kallikrein/ovasin/neuropsin; Serine protease TADG14; TADG 14; TADG14; Tumor associated differentially expressed gene 14; anti-KLK8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig
• Clonality: Polyclonal
• Specificity: KLK8 Polyclonal Antibody detects endogenous levels of KLK8 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-KLK8 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human KLK8:51-150/260
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK8) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK8) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK8) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Related Product Information for anti-KLK8 antibody

Kallikrein 8 (hK8), also known as neuropsin, brain serine protease 1 (BSP1), tumor associated differentially expressed gene 14 (TADG14), PRSS19, HNP and ovasin, is a trypsin like serine protease. Originally described from mouse brain, KLK8 was implicated in neural plasticity and learning. Kallikrein 8 is thought to be involved in neurite outgrowth and fasciculation during nervous system development. Kallikrein 8 is induced in neurite injury, and Kallikrein 8 has been shown to be elevated in the hippocampus on Alzheimer's patients' brains. Animal models of autoimmune encephalomyelitis (EAE) show elevated Kallikrein 8 production. Kallikrein 8 null mice have aberrant synapses in their hippocampus, compared to wild type mice, and are predisposed to seizures. Kallikrein 8 is thought to regulate the early phase of long-term potentiation (LTP), possibly by cleaving fibronectin and other substrates. Kallikrein 8 is also found in the uterus, where it is hormonally regulated. Cervical cancer cell lines express high levels of Kallikrein 8, and it may be useful as a tumor marker. Kallikrein 8 is not limited to the brain and uterus, as many tissues and cell lines make the protein. Endogenous inhibitors include serine protease inhibitor 3 and protein C inhibitor, although Kallikrein 8 can be found complexed to a number of different protease inhibitors.

NCBI and Uniprot Product Information

• NCBI GI #: 1024846618
• NCBI GeneID: 259277
• NCBI Accession #: NP_001311327.1
• NCBI GenBank Nucleotide #: NM_001324398.1
• UniProt Accession #: Q61955; Q8K5D7
• Molecular Weight: 28,524 Da
• NCBI Official Full Name: kallikrein-8 preproprotein
• NCBI Official Synonym Full Names: kallikrein related-peptidase 8
• NCBI Official Symbol: Klk8
• NCBI Official Synonym Symbols: NP; BSP1; Nrpn; Prss19
• NCBI Protein Information: kallikrein-8
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: Klk8
• UniProt Synonym Gene Names: Nrpn; Prss19; mK8; NP

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic cleavage of the activation peptide to generate the functional enzyme. Mice lacking the encoded protein exhibit impaired long-term potentiation and increased anxiety, as well as a hyperkeratosis phenotype. This gene is located in a cluster of several related kallikrein genes on chromosome 7. [provided by RefSeq, May 2016]

Uniprot Description

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 3.4.21.118; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7 B3|7 28.26 cM

Cellular Component: cytoplasm; extracellular space

Molecular Function: serine-type endopeptidase activity

Biological Process: cell death; keratinocyte proliferation; memory; negative regulation of axon regeneration; negative regulation of myelination; neurite morphogenesis; regulation of synapse organization and biogenesis; response to wounding; synapse organization and biogenesis