Human KLK8/Kallikrein-8 PicoKine ELISA Kit | KLK8 elisa kit

Human KLK8/Kallikrein-8 PicoKine ELISA Kit

Cat. Num. AAA177588

• Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
• Reactivity: Human
• Synonyms: KLK8/Kallikrein-8 PicoKine; Human KLK8/Kallikrein-8 PicoKine ELISA Kit; Kallikrein-8; Brain serine protease 1; BSP1; hK8; HNP; HPN; Kallikrein 8 (neuropsin/ovasin); Kallikrein related peptidase 8; Kallikrein8; KLK 8; Klk8; KLK8 protein type 1; KLK8 protein type 2; KLK8_HUMAN; Neuropsin; neuropsin type 1; Neuropsin type 1; included; Neuropsin type 2; mouse; homolog of; NP; NRPN; Ovasin; PRO322; Protease serine 19; PRSS 19; PRSS19; Serine protease 19; Serine protease kallikrein; Serine protease kallikrein/ovasin/neuropsin; Serine protease TADG 14; Serine protease TADG-14; Serine protease TADG14; TADG 14; TADG14; Tumor associated differentially expressed gene 14; Tumor associated differentially expressed gene 14 protein; Tumor-associated differentially expressed gene 14 protein; UNQ283 ; kallikrein-related peptidase 8; KLK8 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human KLK8
• Sequence Length: 139

• Assay Range: 156pg/ml-10000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human KLK8 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, Q29-G260
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human KLK8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK8 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|1468|3882|6352|1625|4190|6526; Standard deviation|67.53|260.1|349.4|95.9|301.7|417.7; CV(%)|4.6|6.7|5.5|5.9|7.2|6.4
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.002|0.115|0.221|0.408|0.692|1.106|1.755|2.485
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human KLK8/Kallikrein-8 PicoKine ELISA Kit standard curve )

Related Product Information for KLK8 elisa kit

Principle of the assay: Human KLK8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK8 has been precoated onto 96-well plates. Standards(NSO, Q29-G260) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK8 amount of sample captured in plate.

Background: Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 527121930
• NCBI GeneID: 11202
• NCBI Accession #: NP_001268360.1
• NCBI GenBank Nucleotide #: NM_001281431.1
• UniProt Accession #: O60259; Q5V9X1; Q5V9X2; Q8IW69; Q9HCB3; Q9NR68; Q9NR69; Q9UIL9; Q9UQ47
• Molecular Weight: 3,653 Da
• NCBI Official Full Name: kallikrein-8 isoform 5
• NCBI Official Synonym Full Names: kallikrein related peptidase 8
• NCBI Official Symbol: KLK8
• NCBI Official Synonym Symbols: NP; HNP; NRPN; PRSS19; TADG14
• NCBI Protein Information: kallikrein-8
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: KLK8
• UniProt Synonym Gene Names: NRPN; PRSS19; TADG14; hK8; NP
• UniProt Entry Name: KLK8_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Secreted; Cell development/differentiation; EC 3.4.21.118; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: cytoplasm; extracellular space

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell death; keratinocyte proliferation; memory; negative regulation of axon regeneration; negative regulation of myelination; neurite morphogenesis; proteolysis; regulation of synapse organization and biogenesis; response to wounding; synapse organization and biogenesis