Kallikrein 8 Active Protein | KLK8 active protein

Kallikrein 8 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120978

• Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Kallikrein 8; Kallikrein 8 Protein; Human; Recombinant (His Tag); Human KLK-8/Kallikrein-8 Protein (His Tag); HNP Protein; NP Protein; NRPN Protein; PRSS19 Protein; TADG14 Protein; kallikrein-related peptidase 8; KLK8 active protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly260

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human KLK8 isoform 1 (O60259-1)(Met 1-Gly 260) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is >400pmoles/min/ug
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KLK8 active protein

Background: Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, and KLK8 is a secreted protein that belongs to the peptidase S1 family and Kallikrein subfamily. It is a serine protease that is capable of degrading some proteins such as casein, fibrinogen, kininogen, fibronectin, and collagen type IV. Kallikrein-8/KLK8 plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. It regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. It is involved in skin desquamation and keratinocyte proliferation and plays a role in the secondary phase of pathogenesis following spinal cord injury. It also cleaves L1CAM in response to increased neural activity. It induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Kallikrein-8/KLK8 is expressed at high levels in serum, ascites fluid, and tumor cytosol of advanced-stage ovarian cancer patients and may serve as a marker of ovarian cancer. Kallikrein-8/KLK8 may have potential clinical value for disease test or prognosis and it may also be a useful therapeutic target.

Product Categories/Family for KLK8 active protein

Serine Proteases and Regulators

References

Yoshida S., et al.,(1998), Sequence analysis and expression of human neuropsin cDNA and gene. Gene 213:9-16.Underwood L.J., et al., (1999), Cloning of tumor-associated differentially expressed gene-14, a novel serine protease overexpressed by ovarian carcinoma.Cancer Res. 59:4435-4439.Mitsui S., et al.,(1999), A novel form of human neuropsin, a brain-related serine protease, is generated by alternative splicing and is expressed preferentially in human adult brain.Eur. J. Biochem. 260:627-634.

NCBI and Uniprot Product Information

• NCBI GI #: 6005844
• NCBI GeneID: 11202
• NCBI Accession #: NP_009127.1
• NCBI GenBank Nucleotide #: NM_007196.2
• UniProt Accession #: O60259; Q5V9X1; Q5V9X2; Q8IW69; Q9HCB3; Q9NR68; Q9NR69; Q9UIL9; Q9UQ47
• Molecular Weight: 28,048 Da
• NCBI Official Full Name: kallikrein-8 isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 8
• NCBI Official Symbol: KLK8
• NCBI Official Synonym Symbols: NP; HNP; NRPN; PRSS19; TADG14
• NCBI Protein Information: kallikrein-8; hK8; ovasin; neuropsin type 1; neuropsin type 2; serine protease 19; KLK8 protein type 1; KLK8 protein type 2; serine protease TADG-14; tumor-associated differentially expressed gene 14 protein
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: KLK8
• UniProt Synonym Gene Names: NRPN; PRSS19; TADG14; hK8; NP
• UniProt Entry Name: KLK8_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.118; Protease; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: extracellular space; cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell death; negative regulation of myelination; negative regulation of axon regeneration; synapse organization and biogenesis; response to wounding; regulation of synapse organization and biogenesis; keratinocyte proliferation; proteolysis; neurite morphogenesis; memory