Rabbit anti-Human EXT2 Polyclonal Antibody | anti-EXT2 antibody

EXT2, ID (EXT2, Exostosin-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Multiple exostoses protein 2, Putative tumor suppressor protein EXT2) (Azide free) (HRP)

Cat. Num. AAA6297178

• Gene Names: EXT2; SOTV
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EXT2; Polyclonal Antibody; ID (EXT2; Exostosin-2; Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; Multiple exostoses protein 2; Putative tumor suppressor protein EXT2) (Azide free) (HRP); Putative tumor suppressor protein EXT2; anti-EXT2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EXT2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188~218 amino acids from the Central region of human EXT2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of EXT2 Antibody (Center) in MDA-MB468 cell line lysates (35ug/lane). EXT2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EXT2 Antibody (Center) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(EXT2 Antibody (Center) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EXT2 antibody

EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.

Product Categories/Family for anti-EXT2 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Zhao, J., et al. Diabetes 59(3):751-755(2010). Li, Y., et al. Genet Test Mol Biomarkers 13(6):825-830(2009). Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Heinritz, W., et al. Ann. Hum. Genet. 73 (PT 3), 283-291 (2009). Cornelis, M.C., et al. Ann. Intern. Med. 150(8):541-550(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 296010873
• NCBI GeneID: 2132
• NCBI Accession #: NP_000392
• NCBI GenBank Nucleotide #: NM_000401
• UniProt Accession #: Q93063; O15288; B2R5Z6; C9JU51; J3KPT2
• Molecular Weight: 85,815 Da
• NCBI Official Full Name: exostosin-2 isoform 1
• NCBI Official Synonym Full Names: exostosin glycosyltransferase 2
• NCBI Official Symbol: EXT2
• NCBI Official Synonym Symbols: SOTV
• NCBI Protein Information: exostosin-2; N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase; glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; multiple exostoses protein 2; putative tumor suppresso
• UniProt Protein Name: Exostosin-2
• Protein Family: Extensin
• UniProt Gene Name: EXT2
• UniProt Entry Name: EXT2_HUMAN