Rabbit anti-Human KLHL3 Polyclonal Antibody | anti-KLHL3 antibody

KLHL3, ID (KLHL3, KIAA1129, Kelch-like protein 3) (Biotin)

Cat. Num. AAA6314185

• Gene Names: KLHL3; PHA2D
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLHL3; Polyclonal Antibody; ID (KLHL3; KIAA1129; Kelch-like protein 3) (Biotin); Kelch-like protein 3; anti-KLHL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 587

• Applicable Applications for anti-KLHL3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLHL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-234 amino acids from the Central region of human KLHL3.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KLHL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KLHL3 antibody detected the KLHL3 protein (arrow).)

Related Product Information for anti-KLHL3 antibody

Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Product Categories/Family for anti-KLHL3 antibody

Antibodies; KIAA; orf

References

Schmutz, J., et al. Nature 431(7006):268-274(2004). Yeo, A., et al. J. Neurochem. 76(6):1688-1700(2001). Lai, F., et al. Genomics 66(1):65-75(2000). Mizutani, A., et al. J. Biol. Chem. 275(13):9823-9831(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 166235129
• NCBI GeneID: 26249
• NCBI Accession #: NP_059111.2
• NCBI GenBank Nucleotide #: NP_059111.2
• UniProt Accession #: Q9UH77
• NCBI Official Full Name: kelch-like protein 3 isoform 1
• NCBI Official Synonym Full Names: kelch like family member 3
• NCBI Official Symbol: KLHL3
• NCBI Official Synonym Symbols: PHA2D
• NCBI Protein Information: kelch-like protein 3
• UniProt Protein Name: Kelch-like protein 3
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL3
• UniProt Synonym Gene Names: KIAA1129
• UniProt Entry Name: KLHL3_HUMAN

NCBI Description

This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]