Western Blot (WB)
(KLHL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KLHL3 antibody detected the KLHL3 protein (arrow).)
Rabbit anti-Human KLHL3 Polyclonal Antibody | anti-KLHL3 antibody
KLHL3, ID (KLHL3, KIAA1129, Kelch-like protein 3)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(KLHL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KLHL3 antibody detected the KLHL3 protein (arrow).)
Western Blot (WB)
(KLHL3 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the KLHL3 antibody detected the KLHL3 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene is ubiquitously expressed and encodes a full-length protein which has an N-terminal BTB domain followed by a BACK domain and six kelch-like repeats in the C-terminus. These kelch-like repeats promote substrate ubiquitination of bound proteins via interaction of the BTB domain with the CUL3 (cullin 3) component of a cullin-RING E3 ubiquitin ligase (CRL) complex. Muatations in this gene cause pseudohypoaldosteronism type IID (PHA2D); a rare Mendelian syndrome featuring hypertension, hyperkalaemia and metabolic acidosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Uniprot Description
KLHL3: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex may act by mediating ubiquitination of SLC12A3/NCC, thereby regulating SLC12A3/NCC subcellular location at the cell membrane. Defects in KLHL3 are the cause of Pseudohypoaldosteronism type 2D (PHA2D). A disorder characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics. PHA2D inheritance is autosomal dominant or recessive. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: cytoskeleton; cytosol
Molecular Function: protein binding; structural molecule activity; actin binding
Biological Process: ion homeostasis; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination
Disease: Pseudohypoaldosteronism, Type Iid
Research Articles on KLHL3
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Product Notes
The KLHL3 klhl3 (Catalog #AAA644894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLHL3, ID (KLHL3, KIAA1129, Kelch-like protein 3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLHL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the KLHL3 klhl3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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