Rabbit anti-Human, Mouse KLHL12 Polyclonal Antibody | anti-KLHL12 antibody

KLHL12, CT (KLHL12, C3IP1, Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog) (APC)

Cat. Num. AAA6314118

• Gene Names: KLHL12; DKIR; C3IP1; hDKIR
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLHL12; Polyclonal Antibody; CT (KLHL12; C3IP1; Kelch-like protein 12; CUL3-interacting protein 1; DKIR homolog) (APC); DKIR homolog; anti-KLHL12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KLHL12 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLHL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 375-404 amino acids from the C-terminal region of human KLHL12.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KLHL12 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).)

Western Blot (WB)

(KLHL12 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).)

Related Product Information for anti-KLHL12 antibody

Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.

Product Categories/Family for anti-KLHL12 antibody

Antibodies; Kelch-like Proteins

References

Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009). Rondou, P., et al. J. Biol. Chem. 283(17):11083-11096(2008). Lim, J., et al. Cell 125(4):801-814(2006). Angers, S., et al. Nat. Cell Biol. 8(4):348-357(2006). Uchida, K., et al. Immunology 116(1):53-63(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 11056006
• NCBI GeneID: 59349
• NCBI Accession #: NP_067646.1
• NCBI GenBank Nucleotide #: NM_021633.2
• UniProt Accession #: Q53G59; Q9HBX5; A6NEN8; B7Z7B8
• Molecular Weight: 67,727 Da
• NCBI Official Full Name: kelch-like protein 12
• NCBI Official Synonym Full Names: kelch-like family member 12
• NCBI Official Symbol: KLHL12
• NCBI Official Synonym Symbols: DKIR; C3IP1; hDKIR
• NCBI Protein Information: kelch-like protein 12; DKIR homolog; kelch-like 12; kelch-like protein C3IP1; CUL3-interacting protein 1
• UniProt Protein Name: Kelch-like protein 12
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL12
• UniProt Synonym Gene Names: C3IP1; hDKIR
• UniProt Entry Name: KLH12_HUMAN

NCBI Description

This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

KLHL12: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: Golgi membrane; ER to Golgi transport vesicle; cytosol

Molecular Function: protein binding

Biological Process: COPII coating of Golgi vesicle; protein monoubiquitination; ER to Golgi vesicle-mediated transport; Wnt receptor signaling pathway