anti-Human CNGB3 Antibody | anti-CNGB3 antibody

Goat anti-CNGB3 (aa175-186) Antibody

Cat. Num. AAA423793

• Gene Names: CNGB3; ACHM1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CNGB3; Goat anti-CNGB3 (aa175-186) Antibody; cyclic nucleotide gated channel beta 3; ACHM1; CNG channel beta-3; cone photoreceptor cGMP-gated cation channel beta-subunit; cyclic nucleotide-gated cation channel beta-3; cyclic nucleotide-gated cation channel modulatory subunit; anti-CNGB3 antibody

• Reactivity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 809

• Applicable Applications for anti-CNGB3 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:8000.; IHC: In paraffin embedded Human Retina shows primarily staining of the pigmented epithelial cells. Recommended concentration: 2-4ug/ml.
• Immunogen Sequence: Peptide with sequence C-ESDDKPTEHYYR, from the internal region of the protein sequence according to NP_061971.3.
• Peptide Sequence + C: C-ESDDKPTEHYYR
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2ug/ml) staining of paraffin embedded Human Retina. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)

Immunohistochemistry (IHC)

(Negative Control showing staining of paraffin embedded Human Retina, with no primary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 116642889
• NCBI GeneID: 54714
• NCBI Accession #: NP_061971.3
• NCBI GenBank Nucleotide #: NM_019098.4
• UniProt Accession #: Q9NQW8
• Molecular Weight: 91,633 Da
• NCBI Official Full Name: cyclic nucleotide-gated cation channel beta-3
• NCBI Official Synonym Full Names: cyclic nucleotide gated channel beta 3
• NCBI Official Symbol: CNGB3
• NCBI Official Synonym Symbols: ACHM1
• NCBI Protein Information: cyclic nucleotide-gated cation channel beta-3
• UniProt Protein Name: Cyclic nucleotide-gated cation channel beta-3
• Protein Family: Cyclic nucleotide-gated cation channel
• UniProt Gene Name: CNGB3
• UniProt Synonym Gene Names: CNG channel beta-3

NCBI Description

This gene encodes the beta subunit of a cyclic nucleotide-gated ion channel. The encoded beta subunit appears to play a role in modulation of channel function in cone photoreceptors. This heterotetrameric channel is necessary for sensory transduction, and mutations in this gene have been associated with achromatopsia 3, progressive cone dystrophy, and juvenile macular degeneration, also known as Stargardt Disease. [provided by RefSeq, Feb 2010]

Uniprot Description

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficiency of the channel when coexpressed with CNGA3 (). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.