Rabbit kininogen 1 Polyclonal Antibody | anti-KNG1 antibody
Rabbit anti-human kininogen 1 polyclonal Antibody
ELISA: Use at an assay dependent dilution.
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100 (Recommender dilutions)
Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, MCF7 cells
Positive IHC detected in: Human endometrial cancer tissue
Positive IF detected in: HepG2 cells
NCBI and Uniprot Product Information
NCBI Description
This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Uniprot Description
KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Inhibitor; Contractile; Cell adhesion; Motility/polarity/chemotaxis; Secreted
Chromosomal Location of Human Ortholog: 3q27
Cellular Component: extracellular space; extracellular region; plasma membrane
Molecular Function: heparin binding; protein binding; zinc ion binding; cysteine protease inhibitor activity; receptor binding
Biological Process: negative regulation of proteolysis; platelet activation; elevation of cytosolic calcium ion concentration; smooth muscle contraction; platelet degranulation; positive regulation of apoptosis; negative regulation of blood coagulation; negative regulation of cell adhesion; inflammatory response; blood coagulation; vasodilation; blood coagulation, intrinsic pathway
Disease: High Molecular Weight Kininogen Deficiency
Research Articles on KNG1
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Product Notes
The KNG1 kng1 (Catalog #AAA710883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human kininogen 1 polyclonal Antibody reacts with Human; Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. AAA Biotech's kininogen 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Tested applications: ELISA: Use at an assay dependent dilution. WB: 1:200-1:2000 IHC: 1:20-1:200 IF: 1:10-1:100 (Recommender dilutions) Positive WB detected in: HeLa cells, A2780 cells, COLO 320 cells, MCF7 cells Positive IHC detected in: Human endometrial cancer tissue Positive IF detected in: HepG2 cells. Researchers should empirically determine the suitability of the KNG1 kng1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "kininogen 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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