Kininogen-1 ELISA Kit | KNG1 elisa kit

Human Kininogen-1/KNG1 PicoKine ELISA Kit

Cat. Num. AAA177545

• Gene Names: KNG1; BK; BDK; KNG
• Reactivity: Human. There is no detectable cross-reactivity with other relevant proteins.
• Synonyms: Kininogen-1; Human Kininogen-1/KNG1 PicoKine ELISA Kit; Alpha-2-thiol proteinase inhibitor; BDK; BK; included; bradykinin; Fitzgerald factor; Flaujeac factor; High molecular weight kininogen; HMWK; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; kininogen 1; KNG; KNG1; KNG1_HUMAN; LMWK; Low molecular weight growth-promoting factor; Low molecular weight kininogen; Williams factor; Williams-Fitzgerald-Flaujeac factor; KNG1 elisa kit

• Reactivity: Human. There is no detectable cross-reactivity with other relevant proteins.
• Sequence Length: 427

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Sensitivity: <20pg/ml
• Immunogen: NSO, Q19-S644
• Intra-Assay Precision: Sample 1: n=16; Mean(ng/ml): 8.5; Standard deviation: 0.323; CV(%): 3.8 Sample 2: n=16; Mean(ng/ml): 14; Standard deviation: 0.658; CV(%): 4.7 Sample 3: n=16; Mean(ng/ml): 28; Standard deviation: 1.54; CV(%): 5.5
• Inter-Assay Precision: Sample 1: n=24; Mean(ng/ml): 10; Standard deviation: 0.43; CV(%): 4.3 Sample 2: n=24; Mean(ng/ml): 19; Standard deviation: 0.969; CV(%): 5.1 Sample 3: n=24; Mean(ng/ml): 35; Standard deviation: 2.17; CV(%): 6.2
• Assay Range: 0.78ng/ml-50ng/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Kininogen-1/KNG1 ELISA Kit standard curve )

Related Product Information for KNG1 elisa kit

Description: Sandwich ELISA kit of Quantitative Detection for Human Kininogen-1/KNG1
Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.

NCBI and Uniprot Product Information

• NCBI GI #: 4504893
• NCBI GeneID: 3827
• NCBI Accession #: NP_000884.1
• NCBI GenBank Nucleotide #: NM_000893.3
• UniProt Accession #: P01042; P01043; Q53EQ0; Q6PAU9; Q7M4P1; A8K474; B2RCR2; C9JEX1
• Molecular Weight: 43,822 Da
• NCBI Official Full Name: kininogen-1 isoform 2
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: KNG1
• NCBI Official Synonym Symbols: BK; BDK; KNG
• NCBI Protein Information: kininogen-1; HMWK; bradykinin; fitzgerald factor; high molecular weight kininogen; alpha-2-thiol proteinase inhibitor; williams-Fitzgerald-Flaujeac factor
• UniProt Protein Name: Kininogen-1
• Protein Family: Kininogen
• UniProt Gene Name: KNG1
• UniProt Synonym Gene Names: BDK; KNG; HMWK
• UniProt Entry Name: KNG1_HUMAN

NCBI Description

This gene uses alternative splicing to generate two different proteins- high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. Bradykinin also functions as an antimicrobial peptide with antibacterial and antifungal activity. In contrast to HMWK, LMWK is not involved in blood coagulation. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Nov 2014]

Uniprot Description

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Contractile; Secreted; Cell adhesion; Inhibitor; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space; extracellular region; plasma membrane

Molecular Function: heparin binding; protein binding; zinc ion binding; cysteine protease inhibitor activity; receptor binding

Biological Process: negative regulation of proteolysis; platelet activation; elevation of cytosolic calcium ion concentration; platelet degranulation; smooth muscle contraction; positive regulation of apoptosis; negative regulation of blood coagulation; negative regulation of cell adhesion; blood coagulation; inflammatory response; vasodilation; blood coagulation, intrinsic pathway

Disease: High Molecular Weight Kininogen Deficiency