Western Blot (WB)
(Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: K562)
Rabbit SF2 Monoclonal Antibody | anti-SF2 antibody
SF2 Antibody
IHC: 1:50-1:200
ICC: 1:50
FC/FACS: 1:50-1:100
Western Blot (WB)
(Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: K562)
Western Blot (WB)
(Western blot analysis of SF2 on different lysates using anti-SF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: K562)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-SF2 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining SF2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of K562 cells with SF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
Calculated MW: 22kDa/27kDa/31kDa
NCBI Description
This gene encodes a member of the arginine/serine-rich splicing factor protein family. The encoded protein can either activate or repress splicing, depending on its phosphorylation state and its interaction partners. Multiple transcript variants have been found for this gene. There is a pseudogene of this gene on chromosome 13. [provided by RefSeq, Jun 2014]
Uniprot Description
SF2: a serine-arginine-rich splicing regulatory protein. Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5' and 3' splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice-site-containing pre-mRNA. Extensively phosphorylated on serine residues in the serine-arginine rich region. Three splice variant isoforms have been described. ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.
Protein type: RNA-binding; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 17q22
Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus
Molecular Function: mRNA binding; protein binding; RS domain binding; RNA binding; nucleotide binding
Biological Process: transcription from RNA polymerase II promoter; in utero embryonic development; RNA splicing; regulation of mRNA stability; regulation of translation; nuclear mRNA splicing, via spliceosome; nuclear mRNA 5'-splice site recognition; mRNA splice site selection; regulation of nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; regulation of transcription, DNA-dependent; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; cardiac muscle contraction
Research Articles on SF2
Similar Products
Product Notes
The SF2 srsf1 (Catalog #AAA9436590) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SF2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the SF2 srsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SF2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
