Rabbit anti-Human KIF11 Polyclonal Antibody | anti-KIF11 antibody

KIF11, NT (KIF11, EG5, KNSL1, TRIP5, Kinesin-like protein KIF11, Kinesin-like protein 1, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, Thyroid receptor-interacting protein 5) (FITC)

Cat. Num. AAA6313196

• Gene Names: KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KIF11; Polyclonal Antibody; NT (KIF11; EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5) (FITC); Thyroid receptor-interacting protein 5; anti-KIF11 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KIF11 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KIF11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109~139 amino acids from the N-terminal region of human KIF11.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KIF11 Antibody (N-term) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KIF11 Antibody (N-term) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KIF11 antibody

KIF11 belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Product Categories/Family for anti-KIF11 antibody

Antibodies; Receptors

References

Tcherniuk, S., et.al., Biochem. Pharmacol. 79 (6), 864-872 (2010). Parke, C.L., et.al., J. Biol. Chem. 285 (8), 5859-5867 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 13699824
• NCBI GeneID: 3832
• NCBI Accession #: NP_004514.2
• NCBI GenBank Nucleotide #: NM_004523.3
• UniProt Accession #: P52732; Q15716; Q5VWX0; A0AV49; B2RMV3
• Molecular Weight: 119,159 Da
• NCBI Official Full Name: kinesin-like protein KIF11
• NCBI Official Synonym Full Names: kinesin family member 11
• NCBI Official Symbol: KIF11
• NCBI Official Synonym Symbols: EG5; HKSP; KNSL1; MCLMR; TRIP5
• NCBI Protein Information: kinesin-like protein KIF11; TRIP-5; kinesin-like protein 1; TR-interacting protein 5; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5
• UniProt Protein Name: Kinesin-like protein KIF11
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF11
• UniProt Synonym Gene Names: EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
• UniProt Entry Name: KIF11_HUMAN

NCBI Description

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF11: a cytoskeletal protein that belongs to the kinesin-like protein family. BimC subfamily. Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Plays a role in chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Phosphorylation regulates its association with the spindle apparatus.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: spindle pole; kinesin complex; microtubule; membrane; spindle microtubule; cytoplasm; spindle; cytosol

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; protein complex binding; microtubule motor activity; protein kinase binding; ATP binding

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic centrosome separation; cell division; metabolic process; regulation of mitotic centrosome separation; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; spindle organization and biogenesis; microtubule-based movement; chromosome segregation

Disease: Microcephaly With Or Without Chorioretinopathy, Lymphedema, Or Mental Retardation