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Immunocytochemistry/Immunofluorescence (ICC/IF))

Human anti-Human Eg5 Monoclonal Antibody | anti-KIF11 antibody

Anti-Eg5 Antibody (4H3-1F12)

Gene Names
KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Synonyms
Eg5; Monoclonal Antibody; Anti-Eg5 Antibody (4H3-1F12); EG5; HKSP; KNSL1; TRIP5; anti-KIF11 antibody
Ordering
For Research Use Only!
Host
Human
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
4H3-1F12
Specificity
Monoclonal antibody to detect Eg5
Purity/Purification
Affinity purification
Form/Format
Colorless liquid. In PBS (pH7.4) containing with 0.02% sodium azide and 50% glycerol
Applicable Applications for anti-KIF11 antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
IF: 1:200
Immunogen
Recombinant human Eg5 protein fragments expressed in E Coli
Positive Control
WB: MCSF, 293T, Jurkat, HeLa cell lysates; IP: Jurkat cells; IF: HeLa cells
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use
Shelf life: ~12 months

Immunocytochemistry/Immunofluorescence (ICC/IF))

Immunocytochemistry/Immunofluorescence (ICC/IF))

Western Blot (WB)

Western Blot (WB)

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-KIF11 antibody
Eg5 gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
119,159 Da
NCBI Official Full Name
kinesin-like protein KIF11
NCBI Official Synonym Full Names
kinesin family member 11
NCBI Official Symbol
KIF11
NCBI Official Synonym Symbols
EG5; HKSP; KNSL1; MCLMR; TRIP5
NCBI Protein Information
kinesin-like protein KIF11; TRIP-5; kinesin-like protein 1; TR-interacting protein 5; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5
UniProt Protein Name
Kinesin-like protein KIF11
Protein Family
UniProt Gene Name
KIF11
UniProt Synonym Gene Names
EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
UniProt Entry Name
KIF11_HUMAN

NCBI Description

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF11: a cytoskeletal protein that belongs to the kinesin-like protein family. BimC subfamily. Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Plays a role in chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Phosphorylation regulates its association with the spindle apparatus.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: spindle pole; kinesin complex; microtubule; membrane; spindle microtubule; cytoplasm; spindle; cytosol

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; protein complex binding; microtubule motor activity; protein kinase binding; ATP binding

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic centrosome separation; cell division; metabolic process; regulation of mitotic centrosome separation; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; spindle organization and biogenesis; microtubule-based movement; chromosome segregation

Disease: Microcephaly With Or Without Chorioretinopathy, Lymphedema, Or Mental Retardation

Research Articles on KIF11

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Product Notes

The KIF11 kif11 (Catalog #AAA847182) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-Eg5 Antibody (4H3-1F12) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Eg5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:1000 IF: 1:200. Researchers should empirically determine the suitability of the KIF11 kif11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eg5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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