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Testing Data (Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1)(MBS213037))

Mouse CD90 Monoclonal Antibody | anti-CD90 antibody

MOUSE ANTI RAT CD90:Low Endotoxin

Gene Names
Thy1; CD7
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Synonyms
CD90; Monoclonal Antibody; MOUSE ANTI RAT CD90:Low Endotoxin; anti-CD90 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-7
Form/Format
Low Endotoxin
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
161
Applicable Applications for anti-CD90 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/40; Maximum Dilution: 1/80
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1)(MBS213037))

Testing Data (Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1)(MBS213037))

Testing Data

(Staining of rat thymocytes with Mouse anti Rat CD90 (MBS213035))

Testing Data (Staining of rat thymocytes with Mouse anti Rat CD90 (MBS213035))

Testing Data

(Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1): Low Endotoxin)

Testing Data (Staining of rat thymus with Mouse anti Rat CD90 (THY 1.1): Low Endotoxin)

Testing Data

(Staining of rat thymocytes with Mouse anti Rat CD90 (THY1.1): FITC (MBS211030))

Testing Data (Staining of rat thymocytes with Mouse anti Rat CD90 (THY1.1): FITC (MBS211030))

Testing Data

(Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1):FITC (MBS216395))

Testing Data (Staining of rat thymus cells with Mouse anti Rat CD90 (THY-1.1):FITC (MBS216395))

Testing Data

(Staining of rat thymus cells with Mouse anti Rat CD90 (THY1.1):Alexa Fluor 488 )

Testing Data (Staining of rat thymus cells with Mouse anti Rat CD90 (THY1.1):Alexa Fluor 488 )
Related Product Information for anti-CD90 antibody
Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice.Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab' of MRC OX-7 for rat Thy1 is 3 x 109m1 and for mouse Thy1.1 is 3 x 108m-1(1). Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996). This product is routinely tested in flow cytometry on rat thymocytes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
18,172 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
Thy1
NCBI Official Synonym Symbols
CD7
NCBI Protein Information
thy-1 membrane glycoprotein; CD90; Thy-1 protein; Thymus cell surface antigen; thy-1 antigen; thy-1 glycoprotein; thymus cell antigen 1, theta
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
Thy1
UniProt Synonym Gene Names
Thy-1
UniProt Entry Name
THY1_RAT

NCBI Description

cell surface glycoprotein antigen [RGD, Feb 2006]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; cell surface; growth cone; anchored to plasma membrane; focal adhesion; endoplasmic reticulum; apical plasma membrane; dendrite; plasma membrane; cytosol; myelin sheath; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; enzyme binding; protein kinase binding

Biological Process: focal adhesion formation; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; mast cell activation; T cell receptor signaling pathway; cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; retinal cone cell development; negative regulation of fibroblast proliferation; negative regulation of protein kinase activity; negative regulation of axonogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; angiogenesis; negative regulation of cell migration; positive regulation of GTPase activity; negative regulation of apoptosis

Research Articles on CD90

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Product Notes

The CD90 thy1 (Catalog #AAA211261) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD90 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/40; Maximum Dilution: 1/80. Researchers should empirically determine the suitability of the CD90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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