Rabbit MYOD Polyclonal Antibody | anti-MYOD antibody

MYOD (Phospho-Ser200) Antibody

Cat. Num. AAA9508643

• Gene Names: MYOD1; PUM; MYF3; MYOD; bHLHc1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MYOD; Polyclonal Antibody; MYOD (Phospho-Ser200) Antibody; MYF3; MYOD1; Myoblast determination protein 1; Myogenic factor MYF-3; anti-MYOD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: MYOD (Phospho-Ser200) Antibody detects endogenous levels of MYOD1 only when phosphorylated at Ser200.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 320

• Applicable Applications for anti-MYOD antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S200; M:S200; R:S200
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human MYOD around the phosphorylation site of Ser200.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MYOD (Phospho-Ser200) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat cells treated with Ca+ 40nM 30', using MYOD (Phospho-Ser200) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 23111009
• NCBI GeneID: 4654
• NCBI Accession #: NP_002469.2
• NCBI GenBank Nucleotide #: NM_002478.4
• UniProt Accession #: P15172; O75321
• Molecular Weight: 34 kDa
• NCBI Official Full Name: myoblast determination protein 1
• NCBI Official Synonym Full Names: myogenic differentiation 1
• NCBI Official Symbol: MYOD1
• NCBI Official Synonym Symbols: PUM; MYF3; MYOD; bHLHc1
• NCBI Protein Information: myoblast determination protein 1
• UniProt Protein Name: Myoblast determination protein 1
• Protein Family: Myosin
• UniProt Gene Name: MYOD1
• UniProt Synonym Gene Names: BHLHC1; MYF3; MYOD; bHLHc1; Myf-3

NCBI Description

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins ().