Western Blot (WB)
(KCTD10 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KCTD10 antibody detected the KCTD10 protein (arrow).)
Rabbit anti-Human KCTD10 Polyclonal Antibody | anti-KCTD10 antibody
KCTD10, CT (KCTD10, ULR061, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3, BTB/POZ domain-containing protein KCTD10, Potassium channel tetramerization domain-containing protein 10) (APC)
Gene Names
KCTD10; BTBD28; ULRO61; MSTP028; hBACURD3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KCTD10; Polyclonal Antibody; CT (KCTD10; ULR061; BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3; BTB/POZ domain-containing protein KCTD10; Potassium channel tetramerization domain-containing protein 10) (APC); Potassium channel tetramerization domain-containing protein 10; anti-KCTD10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-KCTD10 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KCTD10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human KCTD10.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(KCTD10 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KCTD10 antibody detected the KCTD10 protein (arrow).)
Western Blot (WB)
(KCTD10 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the KCTD10 antibody detected the KCTD10 protein (arrow).)
Immunohistochemistry (IHC)
(KCTD10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCTD10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(KCTD10 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCTD10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(KCTD10 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(KCTD10 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-KCTD10 antibody
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex. The BCR(BACURD3) E3 ubiquitin ligase complex mediates the ubiquitination of target proteins, leading to their degradation by the proteasome (By similarity).
Product Categories/Family for anti-KCTD10 antibody
References
Keebler, M.E., et al. Circ Cardiovasc Genet 3(4):358-364(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Junyent, M., et al. Am. J. Clin. Nutr. 90(3):686-694(2009). Wang, Y., et al. J. Cell. Biochem. 106(3):409-413(2009). Liu, R., et al. FEBS J. 276(4):1114-1124(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,432 Da
NCBI Official Full Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3
NCBI Official Synonym Full Names
potassium channel tetramerization domain containing 10
NCBI Official Symbol
KCTD10
NCBI Official Synonym Symbols
BTBD28; ULRO61; MSTP028; hBACURD3
NCBI Protein Information
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3; BTB/POZ domain-containing protein KCTD10; potassium channel tetramerisation domain containing 10; potassium channel tetramerization domain-containing protein 10
UniProt Protein Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3
UniProt Gene Name
KCTD10
UniProt Synonym Gene Names
ULR061; MSTP028; hBACURD3
UniProt Entry Name
BACD3_HUMAN
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Product Notes
The KCTD10 kctd10 (Catalog #AAA6312820) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KCTD10, CT (KCTD10, ULR061, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3, BTB/POZ domain-containing protein KCTD10, Potassium channel tetramerization domain-containing protein 10) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KCTD10 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KCTD10 kctd10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCTD10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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