B7-H3 Recombinant Protein | CD276 recombinant protein

B7-H3 Protein, Human, Recombinant (ECD, hFc Tag)

Cat. Num. AAA8120724

• Gene Names: CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• Purity: >95% as determined by SDS-PAGE
• Synonyms: B7-H3; B7-H3 Protein; Human; Recombinant (ECD; hFc Tag); Human B7-H3/CD276 Protein (ECD; Fc Tag); 4Ig-B7-H3 Protein; B7H3 Protein; B7RP-2 Protein; CD276 molecule; CD276 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr461

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Leu 29
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human CD276 (Q5ZPR3-1)(Met1-Thr461) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CD276 recombinant protein

Background: B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Product Categories/Family for CD276 recombinant protein

Cancer Drug Targets

References

Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.

NCBI and Uniprot Product Information

• NCBI GI #: 67188443
• NCBI GeneID: 80381
• NCBI Accession #: NP_001019907.1
• NCBI GenBank Nucleotide #: NM_001024736.1
• UniProt Accession #: Q5ZPR3; Q6P5Y4; Q6UXI2; Q8NBI8; Q8NC34; Q8NCB6; Q9BXR1
• Molecular Weight: 57,235 Da
• NCBI Official Full Name: CD276 antigen isoform a
• NCBI Official Synonym Full Names: CD276 molecule
• NCBI Official Symbol: CD276
• NCBI Official Synonym Symbols: B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• NCBI Protein Information: CD276 antigen; B7 homolog 3; costimulatory molecule
• UniProt Protein Name: CD276 antigen
• Protein Family: CD276 antigen
• UniProt Gene Name: CD276
• UniProt Synonym Gene Names: B7H3; B7-H3
• UniProt Entry Name: CD276_HUMAN

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; positive regulation of osteoblast differentiation; regulation of immune response; negative regulation of T cell proliferation; T cell activation; negative regulation of inflammatory response; negative regulation of interleukin-2 biosynthetic process; positive regulation of T cell proliferation; immune response; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; negative regulation of interferon-gamma biosynthetic process