Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS642587. This demonstrates that MBS642587 detected the CD69 protein (arrow).)
Rabbit anti-Human CD69 Polyclonal Antibody | anti-CD69 antibody
CD69, ID (CD69, CLEC2C, Early activation antigen CD69, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69) (AP)
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
CD69; Polyclonal Antibody; ID (CD69; CLEC2C; Early activation antigen CD69; Activation inducer molecule; BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; CD69) (AP); anti-CD69 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human CD69.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CD69 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 119-148 in the central region of human CD69, UniProt Accession #Q07108.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS642587. This demonstrates that MBS642587 detected the CD69 protein (arrow).)
Western Blot (WB)
(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS642587. This demonstrates that MBS642587 detected the CD69 protein (arrow).)
Testing Data
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Testing Data
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Related Product Information for anti-CD69 antibody
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-CD69 antibody
References
1. Davila, S., et al. Genes Immun. (2010). In press: 2. Kolenko, P., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 (PT 12), 1258-1260 (2009). 3. Hu, M., et al. J. Mol. Recognit. 22(6):516-520(2009). 4. Radstake, T.R., et al. PLoS ONE 4 (6), E5981 (2009). 5. Natarajan, K., et al. Biochemistry 39(48):14779-14786(2000). 6. Vance, B.A., et al. Arch. Biochem. Biophys. 368(2):214-220(1999). 7. Lopez-Cabrera, M., et al. J. Biol. Chem. 270(37):21545-21551(1995) 8. Bezouska, K., et al. Biochem. Biophys. Res. Commun. 08(1):68-74(1995) 9. Santis, A.G., et al. Eur. J. Immunol. 24(7):1692-1697(1994) 10. Lopez-Cabrera, M., et al. J. Exp. Med. 178(2):537-547(1993) 11. Ziegler, S.F., et al. Eur. J. Immunol. 23(7):1643-1648(1993) 12. Cambiaggi, C., et al. Immunogenetics 36(2):117-120(1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,559 Da
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
Uniprot Description
Function: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Subunit structure: Homodimer; disulfide-linked. Ref.6 Ref.7 Ref.8
Subcellular location: Membrane; Single-pass type II membrane protein.
Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
Developmental stage: Earliest inducible cell surface glycoprotein acquired during lymphoid activation.
Induction: By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.
Post-translational modification: Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
Sequence similarities: Contains 1 C-type lectin domain.
Similar Products
Product Notes
The CD69 cd69 (Catalog #AAA6283379) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD69, ID (CD69, CLEC2C, Early activation antigen CD69, Activation inducer molecule, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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