Rabbit anti-Human KCNA2 Polyclonal Antibody | anti-KCNA2 antibody

KCNA2, CT (KCNA2, Potassium voltage-gated channel subfamily A member 2, NGK1, Voltage-gated K(+) channel HuKIV, Voltage-gated potassium channel HBK5, Voltage-gated potassium channel subunit Kv1.2) (Azide free) (HRP)

Cat. Num. AAA6312218

• Gene Names: KCNA2; HK4; MK2; HBK5; NGK1; RBK2; HUKIV; KV1.2; EIEE32
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KCNA2; Polyclonal Antibody; CT (KCNA2; Potassium voltage-gated channel subfamily A member 2; NGK1; Voltage-gated K(+) channel HuKIV; Voltage-gated potassium channel HBK5; Voltage-gated potassium channel subunit Kv1.2) (Azide free) (HRP); Voltage-gated potassium channel subunit Kv1.2; anti-KCNA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-KCNA2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KCNA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 450-479 amino acids from the C-terminal region of human KCNA2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KCNA2 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KCNA2 antibody detected the KCNA2 protein (arrow).)

Immunohistochemistry (IHC)

(KCNA2 Antibody (C-term) (AP14749b)immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KCNA2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KCNA2 antibody

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes-shaker, shaw, shab, and shal-have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1.

Product Categories/Family for anti-KCNA2 antibody

Antibodies; Ion Channel

References

Denning, E.J., et al. Mol. Membr. Biol. 26(8):397-421(2009). Peters, C.J., et al. Channels (Austin) 3(5):314-322(2009). Martos, V., et al. Proc. Natl. Acad. Sci. U.S.A. 106(26):10482-10486(2009). Stirling, L., et al. Mol. Biol. Cell 20(12):2991-3002(2009). Zhu, J., et al. Biochem. J. 375 (PT 3), 769-775 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4826782
• NCBI GeneID: 3737
• NCBI Accession #: NP_004965.1
• NCBI GenBank Nucleotide #: NM_004974.4
• UniProt Accession #: P16389
• Molecular Weight: 54 kDa
• NCBI Official Full Name: potassium voltage-gated channel subfamily A member 2 isoform a
• NCBI Official Synonym Full Names: potassium voltage-gated channel subfamily A member 2
• NCBI Official Symbol: KCNA2
• NCBI Official Synonym Symbols: HK4; MK2; HBK5; NGK1; RBK2; HUKIV; KV1.2; EIEE32
• NCBI Protein Information: potassium voltage-gated channel subfamily A member 2
• UniProt Protein Name: Potassium voltage-gated channel subfamily A member 2
• Protein Family: Potassium voltage-gated channel subfamily
• UniProt Gene Name: KCNA2
• UniProt Entry Name: KCNA2_HUMAN

NCBI Description

Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

Kv1.2: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.2/KCNA2 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: axon; cell junction; dendrite; endoplasmic reticulum membrane; integral to plasma membrane; lamellipodium; nerve terminal; perikaryon; plasma membrane; presynaptic membrane; voltage-gated potassium channel complex

Molecular Function: delayed rectifier potassium channel activity; potassium channel activity; protein binding; voltage-gated potassium channel activity

Biological Process: generation of action potential; optic nerve structural organization; potassium ion transport; protein homooligomerization; regulation of circadian sleep/wake cycle, non-REM sleep; regulation of dopamine secretion; sensory perception of pain

Disease: Epileptic Encephalopathy, Early Infantile, 32