Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Kallikrein 7 (KLK7) Polyclonal Antibody | anti-KLK7 antibody
Polyclonal Antibody to Kallikrein 7 (KLK7)
Gene Names
Klk1; Kal; KALA; Klk5; Klk7; Klk1l; Klna3; RGK-7; Klk1c7; RSKG-7; RATKALA
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Kallikrein 7 (KLK7); Polyclonal Antibody; Polyclonal Antibody to Kallikrein 7 (KLK7); anti-KLK7 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KLK7. It has been selected for its ability to recognize KLK7 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-RVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSND LMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT SWIKE
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-RVIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSND LMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT SWIKE
Sequence Length
246
Applicable Applications for anti-KLK7 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant KLK7 (Arg24~Glu255) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060527
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,972 Da
NCBI Official Full Name
kallikrein 1
NCBI Official Synonym Full Names
kallikrein 1
NCBI Official Symbol
Klk1
NCBI Official Synonym Symbols
Kal; KALA; Klk5; Klk7; Klk1l; Klna3; RGK-7; Klk1c7; RSKG-7; RATKALA
NCBI Protein Information
kallikrein 1
UniProt Protein Name
Glandular kallikrein-7, submandibular/renal
Protein Family
UniProt Gene Name
Klk7
UniProt Synonym Gene Names
Klk-7; rGK-7
NCBI Description
serine protease; involved in processing of bioactive peptides [RGD, Feb 2006]
Uniprot Description
KLK7: May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. SCCE cleaves insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines. Belongs to the peptidase S1 family. Kallikrein subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.21.35; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: acrosomal vesicle; apical part of cell; nucleus
Molecular Function: endopeptidase activity; serine-type endopeptidase activity
Biological Process: positive regulation of acute inflammatory response; positive regulation of apoptosis; positive regulation of cell proliferation; proteolysis; response to nutrient
Research Articles on KLK7
Similar Products
Product Notes
The KLK7 klk7 (Catalog #AAA2001990) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Kallikrein 7 (KLK7) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kallikrein 7 (KLK7) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KLK7 klk7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-R VIGGYK CEKNSQPWQV ALYSFTKYLC GGVLIDPSWV ITAAHCSSNN YQVWLGRNNL LEDEPFAQHR LVSQSFPHPD YKPFLMRNHT RKPGDDHSND LMLLHLSQPA DITDGVKVID LPTEEPKVGS TCLASGWGST KPLIWEFPDD LQCVNIHLLS NEKCIKAYKE KVTDLMLCAG ELEGGKDTCT GDSGGPLLCD GVLQGITSWG SVPCAKTNMP AIYTKLIKFT SWIKE. It is sometimes possible for the material contained within the vial of "Kallikrein 7 (KLK7), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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