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Kallikrein 13 Active Protein | KLK13 active protein
Kallikrein 13 Protein, Human, Recombinant (His Tag)
Gene Names
KLK13; KLKL4; KLK-L4
Purity
>95% as determined by SDS-PAGE
Synonyms
Kallikrein 13; Kallikrein 13 Protein; Human; Recombinant (His Tag); Human KLK13/Kallikrein-13 Protein (His Tag); KLK-L4 Protein; KLKL4 Protein; kallikrein-related peptidase 13; KLK13 active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ile262
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gly 18
Tag
C-His
Protein Construction
A DNA sequence encoding the pro-form of human KLK13 (NP_056411.1)(Met 1-Ile 262) was expressed with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >200pmoles/min/ug. (Activation description: The proenzyme needs to be activated by Lysyl-Endopeptidase for an activated form)
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for KLK13 active protein
Background: Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers and is regarded as a novel cancer biomarker. Besides, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.
References
Yousef G.M., et al.,(2000), Identification and characterization of KLK-L4, a new kallikrein-like gene that appears to be down-regulated in breast cancer tissues. J. Biol. Chem. 275:11891-11898.Komatsu N., et al., (2003), Expression and localization of tissue kallikrein mRNAs in human epidermis and appendages.J. Invest. Dermatol. 121:542-549.Andres A.M., et al.,(2007), Understanding the accuracy of statistical haplotype inference with sequence data of known phase.Genet. Epidemiol. 31:659-671.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,986 Da
NCBI Official Full Name
kallikrein-13
NCBI Official Synonym Full Names
kallikrein related peptidase 13
NCBI Official Symbol
KLK13
NCBI Official Synonym Symbols
KLKL4; KLK-L4
NCBI Protein Information
kallikrein-13
UniProt Protein Name
Kallikrein-13
Protein Family
UniProt Gene Name
KLK13
UniProt Synonym Gene Names
KLKL4; KLK-L4
UniProt Entry Name
KLK13_HUMAN
Uniprot Description
KLK13: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide; Vesicle
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cytoplasm; extracellular region; extracellular space
Molecular Function: hydrolase activity; protein binding; serine-type endopeptidase activity
Biological Process: protein processing
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Product Notes
The KLK13 klk13 (Catalog #AAA8120091) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ile26 2. It is sometimes possible for the material contained within the vial of "Kallikrein 13, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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