Rabbit JunB Polyclonal Antibody | anti-JunB antibody

JunB (Phospho-Ser259) Antibody

Cat. Num. AAA9408688

• Gene Names: JUNB; AP-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: JunB; Polyclonal Antibody; JunB (Phospho-Ser259) Antibody; AP-1; anti-JunB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of JunB only when phosphorylated at serine 259.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 347

• Applicable Applications for anti-JunB antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 259 (P-V-S(p)-P-I) derived from Human JunB.
• Modification: Phospho-Ser259
• Target Name: JunB
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells untreated or treated with EGF using JunB(Phospho-Ser259) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunB(Phospho-Ser259) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Related Product Information for anti-JunB antibody

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.

Product Categories/Family for anti-JunB antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NM_002229.2
• UniProt Accession #: P17275; Q96GH3
• Molecular Weight: 35,879 Da
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: jun B proto-oncogene
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB
• UniProt Entry Name: JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Oncoprotein; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; double-stranded DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to peptide hormone stimulus; response to light stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; osteoclast differentiation; decidualization; cellular response to hormone stimulus; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; response to mechanical stimulus; transforming growth factor beta receptor signaling pathway; response to cytokine stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; vasculogenesis; positive regulation of cell differentiation; response to progesterone stimulus; response to corticosterone stimulus