Rabbit anti-Human B3GALT5 Polyclonal Antibody | anti-B3GALT5 antibody

B3GALT5, NT (B3GALT5, Beta-1,3-galactosyltransferase 5, Beta-3-Gx-T5, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 5, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 5) (PE)

Cat. Num. AAA6277084

• Gene Names: B3GALT5; B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B3GALT5; Polyclonal Antibody; NT (B3GALT5; Beta-1; 3-galactosyltransferase 5; Beta-3-Gx-T5; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1; 3-galactosyltransferase 5) (PE); anti-B3GALT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 310

• Applicable Applications for anti-B3GALT5 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: B3GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-91 amino acids from the N-terminal region of human B3GALT5.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(B3GALT5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the B3GALT5 antibody detected the B3GALT5 protein (arrow).)

Flow Cytometry (FC/FACS)

(B3GALT5 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-B3GALT5 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Hamshere, M.L., et al. Br J Psychiatry 195(1):23-29(2009). Lin, C.H., et al. Glycobiology 19(4):418-427(2009). Seko, A., et al. Tumour Biol. 30(1):43-50(2009). Mare, L., et al. J. Biol. Chem. 282(1):49-57(2007). Hu, Y.H., et al. BMC Genomics 7, 155 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 15451885
• NCBI GeneID: 10317
• NCBI Accession #: NP_149362.1; NP_149363.1; NP_149360.1; NP_149361.1; NP_006048.1
• NCBI GenBank Nucleotide #: NP_149362.1
• UniProt Accession #: Q9Y2C3
• NCBI Official Full Name: beta-1,3-galactosyltransferase 5
• NCBI Official Synonym Full Names: beta-1,3-galactosyltransferase 5
• NCBI Official Symbol: B3GALT5
• NCBI Official Synonym Symbols: B3T5; GLCT5; B3GalTx; B3GalT-V; beta3Gal-T5; beta-3-Gx-T5; beta-1,3-GalTase 5
• NCBI Protein Information: beta-1,3-galactosyltransferase 5
• UniProt Protein Name: Beta-1,3-galactosyltransferase 5
• Protein Family: Beta-1,3-galactosyltransferase
• UniProt Gene Name: B3GALT5
• UniProt Synonym Gene Names: Beta-1,3-GalTase 5; Beta3Gal-T5; Beta3GalT5; b3Gal-T5
• UniProt Entry Name: B3GT5_HUMAN

NCBI Description

This gene encodes a member of a family of membrane-bound glycoproteins. The encoded protein may synthesize type 1 Lewis antigens, which are elevated in gastrointestinal and pancreatic cancers. Alternatively spliced transcript variants using multiple alternate promoters have been observed for this gene. [provided by RefSeq, Sep 2017]