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Western Blot (WB) ((0.3ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat JUNB Polyclonal Antibody | anti-JUNB antibody

Goat anti-JUNB Antibody

Gene Names
JUNB; AP-1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
JUNB; Polyclonal Antibody; Goat anti-JUNB Antibody; jun B proto-oncogene; AP-1; activator protein 1; JUNB antibody; jun B proto-oncogene antibody; AP-1 antibody; activator protein 1 antibody; anti-JUNB antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TLKAENAGLSSTAG
Sequence Length
347
Applicable Applications for anti-JUNB antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 35kDa band observed in Human Breast cancer lysates (calculated MW of 35.9kDa according to NP_002220.1). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-TLKAENAGLSSTAG, from the internal region of the protein sequence according to NP_002220.1.
Epitope
Internal region
Note
This antibody may also cross-react with human, mouse, rat, cow, dog JUN (GeneID: 3725, 16476, 24516, 280831, 609429).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,879 Da
NCBI Official Full Name
transcription factor jun-B
NCBI Official Synonym Full Names
JunB proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUNB
NCBI Official Synonym Symbols
AP-1
NCBI Protein Information
transcription factor jun-B
UniProt Protein Name
Transcription factor jun-B
Protein Family
UniProt Gene Name
JUNB
UniProt Entry Name
JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: chromatin; nuclear chromatin; nucleoplasm; transcription factor complex

Molecular Function: DNA binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding

Biological Process: cellular response to hormone stimulus; decidualization; embryonic process involved in female pregnancy; negative regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; osteoclast differentiation; positive regulation of cell differentiation; positive regulation of transcription from RNA polymerase II promoter; regulation of cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to light stimulus; response to lipopolysaccharide; response to mechanical stimulus; response to peptide hormone stimulus; response to progesterone stimulus; response to radiation; transcription from RNA polymerase II promoter; trophectodermal cell differentiation; vasculogenesis

Research Articles on JUNB

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Product Notes

The JUNB junb (Catalog #AAA421724) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-JUNB Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's JUNB can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 35kDa band observed in Human Breast cancer lysates (calculated MW of 35.9kDa according to NP_002220.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the JUNB junb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TLKAENAGLS STAG. It is sometimes possible for the material contained within the vial of "JUNB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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