Rabbit anti-Human, Mouse JMJD4 Polyclonal Antibody | anti-JMJD4 antibody

JMJD4, CT (Jumonji Domain-containing Protein 4, JmjC Domain-containing Protein 4)

Cat. Num. AAA627258

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: JMJD4; Polyclonal Antibody; CT (Jumonji Domain-containing Protein 4; JmjC Domain-containing Protein 4); Anti -JMJD4; anti-JMJD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human JMJD4. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-JMJD4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:250-1:500; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the C-terminal region of human JMJD4 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with JMJD4 (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of anti-JMJD4 (C-term) Pab in Hela and mouse kidney cell line lysate. JMJD4 (C-term)(arrow) was detected using the purified Pab.)

Related Product Information for anti-JMJD4 antibody

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Product Categories/Family for anti-JMJD4 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 56204643
• UniProt Accession #: Q9H9V9; Q5TBZ1; Q5TBZ6; Q9H970
• Molecular Weight: 52,493 Da
• NCBI Official Full Name: jumonji domain containing 4
• UniProt Protein Name: JmjC domain-containing protein 4
• Protein Family: JmjC domain-containing protein
• UniProt Gene Name: JMJD4
• UniProt Entry Name: JMJD4_HUMAN

Uniprot Description

JMJD4: 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q42.13

Molecular Function: protein binding