Rabbit anti-Human JMJD3 Polyclonal Antibody | anti-JMJD3 antibody

JMJD3, NT (KDM6B, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B) (PE)

Cat. Num. AAA6311740

• Gene Names: KDM6B; JMJD3
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: JMJD3; Polyclonal Antibody; NT (KDM6B; KIAA0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3; Lysine demethylase 6B) (PE); KDM6B; Lysine demethylase 6B; anti-JMJD3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-JMJD3 antibody: Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:250-500; Applications are based on unconjugated antibody.
• Immunogen: JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human JMJD3.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-JMJD3 (N-term) Pab (AP1022a) in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-JMJD3 antibody

Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.

Product Categories/Family for anti-JMJD3 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 122937251
• NCBI GeneID: 23135
• NCBI Accession #: EAW90126
• NCBI GenBank Nucleotide #: NM_001080424.1
• UniProt Accession #: O15054; Q96G33; C9IZ40
• NCBI Official Full Name: lysine-specific demethylase 6B
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 6B
• NCBI Official Symbol: KDM6B
• NCBI Official Synonym Symbols: JMJD3
• NCBI Protein Information: lysine-specific demethylase 6B; lysine demethylase 6B; jmjC domain-containing protein 3; jumonji domain-containing protein 3; jumonji domain containing 3, histone lysine demethylase
• UniProt Protein Name: Lysine-specific demethylase 6B
• UniProt Gene Name: KDM6B
• UniProt Synonym Gene Names: JMJD3; KIAA0346
• UniProt Entry Name: KDM6B_HUMAN

Uniprot Description

JMJD3: Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Interacts with TLE1. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and KDM6B. By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Belongs to the UTX family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; dioxygenase activity; sequence-specific DNA binding; metal ion binding; beta-catenin binding

Biological Process: endothelial cell differentiation; establishment and/or maintenance of chromatin architecture; mesodermal cell differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; cardiac muscle cell differentiation