Western Blot (WB)
(Western Blot analysis of JMJD2D expression in transfected 293T cell line by JMJD2D polyclonal antibody. Lane 1: JMJD2D transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human JMJD2D Polyclonal Antibody | anti-KDM4D antibody
JMJD2D (Jumonji Domain Containing 2D, JMJD2D, KDM4D, Lysine-specific Demethylase 4D, JmjC Domain-containing Histone Demethylation Protein 3D, JHDM3D, FLJ10251, MGC141909)
Gene Names
KDM4D; JMJD2D
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
JMJD2D; Polyclonal Antibody; JMJD2D (Jumonji Domain Containing 2D; KDM4D; Lysine-specific Demethylase 4D; JmjC Domain-containing Histone Demethylation Protein 3D; JHDM3D; FLJ10251; MGC141909); Anti -JMJD2D (Jumonji Domain Containing 2D; anti-KDM4D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human JMJD2D.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
METMKSKANCAQNPNCNIMIFHPTKEEFNDFDKYIAYMESQGAHRAGLAKIIPPKEWKARETYDNISEILIATPLQQVASGRAGVFTQYHKKKKAMTVGEYRHLANSKKYQTPPHQNFEDLERKYWKNRIYNSPIYGADISGSLFDENTKQWNLGHLGTIQDLLEKECGVVIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHLGEPKTWYVVPPEHGQRLERLARELFPGSSRGCGAFLRHKVALISPTVLKENGIPFNRITQEAGEFMVTFPYGYHAGFNHGFNCAEAINFATPRWIDYGKMASQCSCGEARVTFSMDAFVRILQPERYDLWKRGQDRAVVDHMEPRVPASQELSTQKEVQLPRRAALGLRQLPSHWARHSPWPMAARSGTRCHTLVCSSLPRRSAVSGTATQPRAAAVHSSKKPSSTPSSTPGPSAQIIHPSNGRRGRGRPPQKLRAQELTLQTPAKRPLLAGTTCTASGPEPEPLPEDGALMDKPVPLSPGLQHPVKASGCSWAPVP
Applicable Applications for anti-KDM4D antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human JMJD2D, aa1-523 (NP_060509.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of JMJD2D expression in transfected 293T cell line by JMJD2D polyclonal antibody. Lane 1: JMJD2D transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of JMJD2D expression in transfected 293T cell line by JMJD2D polyclonal antibody. Lane 1: JMJD2D transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-KDM4D antibody
Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
Product Categories/Family for anti-KDM4D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
58,603 Da
NCBI Official Full Name
JMJD2D protein
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4D
NCBI Official Symbol
KDM4D
NCBI Official Synonym Symbols
JMJD2D
NCBI Protein Information
lysine-specific demethylase 4D; jumonji domain containing 2D; jumonji domain-containing protein 2D; jmjC domain-containing histone demethylation protein 3D
UniProt Protein Name
Lysine-specific demethylase 4D
Protein Family
UniProt Gene Name
KDM4D
UniProt Synonym Gene Names
JHDM3D; JMJD2D
UniProt Entry Name
KDM4D_HUMAN
Uniprot Description
JMJD2D: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys- 9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family.
Protein type: Oxidoreductase; EC 1.14.11.-
Chromosomal Location of Human Ortholog: 11q21
Cellular Component: nucleoplasm
Molecular Function: dioxygenase activity; metal ion binding; histone demethylase activity (H3-K9 specific)
Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent
Research Articles on KDM4D
Similar Products
Product Notes
The KDM4D kdm4d (Catalog #AAA6010053) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JMJD2D (Jumonji Domain Containing 2D, JMJD2D, KDM4D, Lysine-specific Demethylase 4D, JmjC Domain-containing Histone Demethylation Protein 3D, JHDM3D, FLJ10251, MGC141909) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the KDM4D kdm4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: METMKSKANC AQNPNCNIMI FHPTKEEFND FDKYIAYMES QGAHRAGLAK IIPPKEWKAR ETYDNISEIL IATPLQQVAS GRAGVFTQYH KKKKAMTVGE YRHLANSKKY QTPPHQNFED LERKYWKNRI YNSPIYGADI SGSLFDENTK QWNLGHLGTI QDLLEKECGV VIEGVNTPYL YFGMWKTTFA WHTEDMDLYS INYLHLGEPK TWYVVPPEHG QRLERLAREL FPGSSRGCGA FLRHKVALIS PTVLKENGIP FNRITQEAGE FMVTFPYGYH AGFNHGFNCA EAINFATPRW IDYGKMASQC SCGEARVTFS MDAFVRILQP ERYDLWKRGQ DRAVVDHMEP RVPASQELST QKEVQLPRRA ALGLRQLPSH WARHSPWPMA ARSGTRCHTL VCSSLPRRSA VSGTATQPRA AAVHSSKKPS STPSSTPGPS AQIIHPSNGR RGRGRPPQKL RAQELTLQTP AKRPLLAGTT CTASGPEPEP LPEDGALMDK PVPLSPGLQH PVKASGCSWA PVP. It is sometimes possible for the material contained within the vial of "JMJD2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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