Dot Blot (DB)
(Dot blot analysis of anti-Phospho-JMJD2B-S566 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Human JMJD2B Polyclonal Antibody | anti-JMJD2B antibody
JMJD2B, Phosphorylated (S566) (KDM4B, JHDM3B, JMJD2B, KIAA0876, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B) (PE)
Gene Names
KDM4B; JMJD2B; TDRD14B
Reactivity
Human
Applications
Western Blot, Dot Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
JMJD2B; Polyclonal Antibody; Phosphorylated (S566) (KDM4B; JHDM3B; KIAA0876; Lysine-specific demethylase 4B; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B) (PE); KDM4B; Jumonji domain-containing protein 2B; anti-JMJD2B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-JMJD2B antibody
Western Blot (WB), FLISA, Dot Blot (DB)
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Phospho-JMJD2B-S566 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding S566 of human JMJD2B.
Conjugate
PE
Antigen Modification
Phosphorylated
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-JMJD2B-S566 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of anti-Phospho-JMJD2B-S566 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-JMJD2B antibody
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
Product Categories/Family for anti-JMJD2B antibody
References
Yang, J., et al. Cancer Res. 70(16):6456-6466(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Beyer, S., et al. J. Biol. Chem. 283(52):36542-36552(2008). Pollard, P.J., et al. Biochem. J. 416(3):387-394(2008). Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121,897 Da
NCBI Official Full Name
lysine-specific demethylase 4B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4B
NCBI Official Symbol
KDM4B
NCBI Official Synonym Symbols
JMJD2B; TDRD14B
NCBI Protein Information
lysine-specific demethylase 4B; tudor domain containing 14B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
KDM4B
UniProt Synonym Gene Names
JHDM3B; JMJD2B; KIAA0876
UniProt Entry Name
KDM4B_HUMAN
Uniprot Description
JMJD2B: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate. Belongs to the JHDM3 histone demethylase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Demethylase; EC 1.14.11.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleoplasm; focal adhesion; nucleus; cell junction
Molecular Function: dioxygenase activity; zinc ion binding
Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin modification
Research Articles on JMJD2B
Similar Products
Product Notes
The JMJD2B kdm4b (Catalog #AAA6311696) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JMJD2B, Phosphorylated (S566) (KDM4B, JHDM3B, JMJD2B, KIAA0876, Lysine-specific demethylase 4B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA, Dot Blot (DB). WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the JMJD2B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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