Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable peptidyl-tRNA hydrolase (PTRH1) Standard Curve.)
Mouse Probable Peptidyl-tRNA Hydrolase (PTRH1) ELISA Kit | PTRH1 elisa kit
Mouse Probable Peptidyl-tRNA Hydrolase (PTRH1) ELISA Kit
Gene Names
Ptrh1; AW046744; 2210013M04Rik
Reactivity
Mouse
Synonyms
Probable Peptidyl-tRNA Hydrolase (PTRH1); Mouse Probable Peptidyl-tRNA Hydrolase (PTRH1) ELISA Kit; PTRH1; C9orf115; MGC51999; PTH1; peptidyl-tRNA hydrolase 1 homolog; PTRH1 elisa kit
Reactivity
Mouse
Specificity
Mouse Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PTRH1. No significant cross-reactivity or interference between Mouse PTRH1 and analogues was observed.
Sequence Length
204
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable peptidyl-tRNA hydrolase (PTRH1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable peptidyl-tRNA hydrolase (PTRH1) Standard Curve.)
Related Product Information for PTRH1 elisa kit
Description: This Mouse Probable peptidyl-tRNA hydrolase (PTRH1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTRH1 in samples. An antibody specific for PTRH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTRH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTRH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTRH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PTRH1, Belongs to the PTH family.Peptidyl-tRNA hydrolase (Pth) activity releases tRNA from the premature translation termination product peptidyl-tRNA. Two different enzymes have been reported to encode such activity, Pth present in bacteria and eukaryotes and Pth2 present in archaea and eukaryotes. Prokaryotic and eukaryotic cells show an enzymatic activity that releases the peptidyl moiety from the tRNA, called peptidyl-tRNA hydrolase (Pth), allowing the free tRNA and peptide to be reused in protein synthesis. First identified in Escherichia coli, genes encoding Pth have been found in bacteria and eukaryotes but not in archaea. E. coli Pth crystal structure has been solved and exhibits an alpha/beta fold formed by three layers with a mixed five-strand beta-sheet at the core.
Background: PTRH1, Belongs to the PTH family.Peptidyl-tRNA hydrolase (Pth) activity releases tRNA from the premature translation termination product peptidyl-tRNA. Two different enzymes have been reported to encode such activity, Pth present in bacteria and eukaryotes and Pth2 present in archaea and eukaryotes. Prokaryotic and eukaryotic cells show an enzymatic activity that releases the peptidyl moiety from the tRNA, called peptidyl-tRNA hydrolase (Pth), allowing the free tRNA and peptide to be reused in protein synthesis. First identified in Escherichia coli, genes encoding Pth have been found in bacteria and eukaryotes but not in archaea. E. coli Pth crystal structure has been solved and exhibits an alpha/beta fold formed by three layers with a mixed five-strand beta-sheet at the core.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable peptidyl-tRNA hydrolase
NCBI Official Synonym Full Names
peptidyl-tRNA hydrolase 1 homolog
NCBI Official Symbol
Ptrh1
NCBI Official Synonym Symbols
AW046744; 2210013M04Rik
NCBI Protein Information
probable peptidyl-tRNA hydrolase
UniProt Protein Name
Probable peptidyl-tRNA hydrolase
Protein Family
UniProt Gene Name
Ptrh1
UniProt Synonym Gene Names
PTH
UniProt Entry Name
PTH_MOUSE
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Product Notes
The Mouse PTRH1 ptrh1 (Catalog #AAA9717216) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717216 ELISA Kit recognizes Mouse PTRH1. It is sometimes possible for the material contained within the vial of "Probable Peptidyl-tRNA Hydrolase (PTRH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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