SERPINF1 active protein

Recombinant Human SERPINF1

Cat. Num. AAA4159702

• Gene Names: SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SERPINF1; Recombinant Human SERPINF1; PEDF; SerpinF1; EPC-1; Cell proliferation-inducing gene 35 protein; PIG35; Recombinant Human Pigment Epithelium-Derived Factor (SERPINF1); SERPINF1 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered 20 mM PB,150 mM NaCl solution pH 7.5.
• Sequence Length: 418

• Bioactivity: The ED(50) was not determined.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Related Product Information for SERPINF1 active protein

Pigment epithelium-derived factor (PEDF), a 50-kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression. PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown. PEDF exerts anti-angiogenic activity by arresting VEGF-or bFGF-mediated endothelial cell migration, inhibiting capillary morphogenesis, and inducing endothelial cell apoptosis. In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase 8, which initiates the downstream apoptotic cascade. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases 8 and 9.

NCBI and Uniprot Product Information

• NCBI GI #: 39725934
• NCBI GeneID: 5176
• NCBI Accession #: NP_002606.3
• NCBI GenBank Nucleotide #: NP_002606.3
• UniProt Accession #: P36955
• Molecular Weight: 45.0 kDa
• NCBI Official Full Name: pigment epithelium-derived factor isoform 1
• NCBI Official Synonym Full Names: serpin family F member 1
• NCBI Official Symbol: SERPINF1
• NCBI Official Synonym Symbols: OI6; OI12; PEDF; EPC-1; PIG35
• NCBI Protein Information: pigment epithelium-derived factor
• UniProt Protein Name: Pigment epithelium-derived factor
• Protein Family: Pigment epithelium-derived factor
• UniProt Gene Name: SERPINF1
• UniProt Synonym Gene Names: PEDF; PEDF
• UniProt Entry Name: PEDF_HUMAN

NCBI Description

This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]

Uniprot Description

PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: axon hillock; extracellular matrix; extracellular space; perinuclear region of cytoplasm; extracellular region; melanosome; basement membrane

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: cell proliferation; positive regulation of neurogenesis; negative regulation of angiogenesis; ovulation cycle; retina development in camera-type eye; short-term memory; response to arsenic; negative regulation of inflammatory response; multicellular organismal development; response to acidity; kidney development; aging

Disease: Osteogenesis Imperfecta, Type Vi