Rabbit anti-Human JHDM1a Polyclonal Antibody | anti-JHDM1a antibody

JHDM1a, ID (JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A, F-box/LRR-repeat protein 11, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, CXXC-type zinc finger protein 8, F

Cat. Num. AAA6483847

• Gene Names: KDM2A; FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA; FLJ00115; FLJ46431; KIAA1004; SF-KDM2A; DKFZp434M1735
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: JHDM1a; Polyclonal Antibody; ID (JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A; F-box/LRR-repeat protein 11; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; CXXC-type zinc finger protein 8; F; anti-JHDM1a antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FBXL11.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-JHDM1a antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide at the Center of human JHDM1a/FBXL11 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with JHDM1a/FBXL11 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of JHDM1a/FBXL11 (arrow) using rabbit polyclonal JHDM1a/FBXL11 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JHDM1a/FBXL11 gene (Lane 2) (Origene Technologies).)

Western Blot (WB)

(JHDM1a/FBXL11 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the JHDM1a/FBXL11 antibody detected the JHDM1a/FBXL11 protein (arrow).)

Product Categories/Family for anti-JHDM1a antibody

Antibodies; Enzymes; Demethylase

References

1. Tsukada, Y., Nature 439(7078), 811-816 (2006). 2. Andersen, J.S., Nature 433(7021), 77-83 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 16306580
• NCBI GeneID: 22992
• NCBI Accession #: NP_036440
• NCBI GenBank Nucleotide #: NM_012308.2
• UniProt Accession #: Q9Y2K7; Q49A21; Q4G0M3; Q69YY8; Q9BVH5; Q9H7H5; Q9UK66
• Molecular Weight: 132,793 Da
• NCBI Official Full Name: lysine-specific demethylase 2A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 2A
• NCBI Official Symbol: KDM2A
• NCBI Official Synonym Symbols: FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA; FLJ00115; FLJ46431; KIAA1004; SF-KDM2A; DKFZp434M1735
• NCBI Protein Information: lysine-specific demethylase 2A; F-box protein FBL7; OTTHUMP00000235168; OTTHUMP00000235169; F-box protein FBL11; F-box protein Lilina; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-r
• UniProt Protein Name: Lysine-specific demethylase 2A
• UniProt Gene Name: KDM2A
• UniProt Synonym Gene Names: CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004
• UniProt Entry Name: KDM2A_HUMAN

Uniprot Description

FBXL11: Histone demethylase that specifically demethylates 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys- 36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Belongs to the JHDM1 histone demethylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Demethylase; EC 1.14.11.27

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus

Molecular Function: unmethylated CpG binding; zinc ion binding; histone demethylase activity (H3-K36 specific)

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; double-strand break repair via nonhomologous end joining