Western Blot (WB)
(Fig: 1 Western blot analysis of Cre using at 1: 2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).)
Rabbit Cre Polyclonal Antibody | anti-Cre antibody
Polyclonal Antibody to Cre
IHC (paraffin): 1: 1000-1: 5000
IP: 1: 50-1: 200
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Fig: 1 Western blot analysis of Cre using at 1: 2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).)
Western Blot (WB)
(Fig: 1 Western blot analysis of Cre using at 1: 2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).)
Immunohistochemistry (IHC)
(Fig: 2 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 2 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.)
Immunohistochemistry (IHC)
(Fig: 3 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.)
Immunohistochemistry (IHC)
(Fig: 3 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
Uniprot Description
TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family.
Protein type: EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein
Chromosomal Location of Human Ortholog: 20q12
Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; DNA topoisomerase type I activity; protein binding
Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; protein sumoylation; response to drug
Research Articles on Cre
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Product Notes
The Cre top1 (Catalog #AAA668606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cre reacts with Cre/lox Systems and may cross-react with other species as described in the data sheet. AAA Biotech's Cre can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). WB: 1: 1000-1: 2000 IHC (paraffin): 1: 1000-1: 5000 IP: 1: 50-1: 200. Researchers should empirically determine the suitability of the Cre top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cre, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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