Rabbit NPM Polyclonal Antibody | anti-NPM antibody

NPM (Phospho-T199) Polyclonal Antibody

Cat. Num. AAA3000044

• Gene Names: NPM1; B23; NPM
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >97% (by SDS-PAGE).
• Synonyms: NPM; Polyclonal Antibody; NPM (Phospho-T199) Polyclonal Antibody; Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; anti-NPM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-NPM (T199) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr199.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >97% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 259

• Applicable Applications for anti-NPM antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 199.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-NPM (T199) pAb at 1:500 dilutionLane1:H1792 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:EC9706 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug) Lane6:CT26 whole cell lysate (40ug) Lane7:HEK293T whole cell lysate (40ug))

Related Product Information for anti-NPM antibody

Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

NCBI and Uniprot Product Information

• NCBI GI #: 83641870
• NCBI GeneID: 4869
• NCBI Accession #: NP_001032827.1
• NCBI GenBank Nucleotide #: NP_001032827.1
• UniProt Accession #: P06748
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: nucleophosmin isoform 3
• NCBI Official Synonym Full Names: nucleophosmin 1
• NCBI Official Symbol: NPM1
• NCBI Official Synonym Symbols: B23; NPM
• NCBI Protein Information: nucleophosmin
• UniProt Protein Name: Nucleophosmin
• UniProt Gene Name: NPM1
• UniProt Synonym Gene Names: NPM; NPM
• UniProt Entry Name: NPM_HUMAN

NCBI Description

The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Oncoprotein; Nucleolus; Translation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: nucleoplasm; centrosome; focal adhesion; membrane; cytoplasm; nucleolus; spindle pole centrosome; cytosol; ribonucleoprotein complex; nucleus

Molecular Function: protein homodimerization activity; protein kinase inhibitor activity; histone binding; RNA binding; transcription coactivator activity; unfolded protein binding; protein kinase binding; ribosomal large subunit binding; NF-kappaB binding; protein binding; protein heterodimerization activity; Tat protein binding; ribosomal small subunit binding

Biological Process: regulation of centriole replication; viral reproduction; positive regulation of translation; centrosome cycle; cell aging; nucleocytoplasmic transport; DNA repair; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; intracellular protein transport; negative regulation of cell proliferation; nucleosome assembly; regulation of endodeoxyribonuclease activity; protein localization; DNA replication-independent nucleosome assembly at centromere; ribosome assembly; response to stress; negative regulation of apoptosis