Rabbit anti-Human ITPA Polyclonal Antibody | anti-ITPA antibody

ITPA, NT (ITPA, C20orf37, Inosine triphosphate pyrophosphatase, Non-canonical purine NTP pyrophosphatase, Non-standard purine NTP pyrophosphatase, Nucleoside-triphosphate diphosphatase, Nucleoside-triphosphate pyrophosphatase, Putative oncogene protein hl

Cat. Num. AAA6311401

• Gene Names: ITPA; My049; C20orf37; dJ794I6.3; HLC14-06-P
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ITPA; Polyclonal Antibody; NT (ITPA; C20orf37; Inosine triphosphate pyrophosphatase; Non-canonical purine NTP pyrophosphatase; Non-standard purine NTP pyrophosphatase; Nucleoside-triphosphate diphosphatase; Nucleoside-triphosphate pyrophosphatase; Putative oncogene protein hl; Putative oncogene protein hlc14-06-p; anti-ITPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ITPA antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: ITPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 30-60 from the N-terminal region of human ITPA.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ITPA Antibody (N-term) in A375 cell line lysates (35ug/lane). ITPA (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(ITPA Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITPA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(ITPA Antibody (N-term) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of ITPA Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-ITPA antibody

ITPA hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. The encoded protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency. Two transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-ITPA antibody

Antibodies; KIAA; orf

References

Fellay, J., et al. Nature 464(7287):405-408(2010). Herting, G., et al. Biochim. Biophys. Acta 1802(2):269-274(2010). Kudo, M., et al. Drug Metab. Pharmacokinet. 24(6):557-564(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 30173120
• NCBI GeneID: 3704
• UniProt Accession #: Q9BY32; O14878; Q5JWH4; Q9BYN1; Q9BYX0; Q9H3H8; A2A2N2; A4UIM5; B2BCH7
• Molecular Weight: 21,446 Da
• NCBI Official Full Name: Inosine triphosphate pyrophosphatase
• NCBI Official Synonym Full Names: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• NCBI Official Symbol: ITPA
• NCBI Official Synonym Symbols: My049; C20orf37; dJ794I6.3; HLC14-06-P
• NCBI Protein Information: inosine triphosphate pyrophosphatase; ITPase; NTPase; inosine triphosphatase-A; putative oncogene protein HLC14-06-P; nucleoside-triphosphate diphosphatase; non-standard purine NTP pyrophosphatase; non-canonical purine NTP pyrophosphatase; inosine triphos
• UniProt Protein Name: Inosine triphosphate pyrophosphatase
• UniProt Gene Name: ITPA
• UniProt Synonym Gene Names: C20orf37; ITPase; Inosine triphosphatase; NTPase
• UniProt Entry Name: ITPA_HUMAN

NCBI Description

This gene encodes an inosine triphosphate pyrophosphohydrolase. The encoded protein hydrolyzes inosine triphosphate and deoxyinosine triphosphate to the monophosphate nucleotide and diphosphate. This protein, which is a member of the HAM1 NTPase protein family, is found in the cytoplasm and acts as a homodimer. Defects in the encoded protein can result in inosine triphosphate pyrophosphorylase deficiency which causes an accumulation of ITP in red blood cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

ITPA: Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPAD). It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme. Belongs to the HAM1 NTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 3.6.1.19; Hydrolase

Chromosomal Location of Human Ortholog: 20p

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; nucleotide binding

Biological Process: deoxyribonucleoside triphosphate catabolic process; nucleobase, nucleoside and nucleotide metabolic process; ITP catabolic process; chromosome organization and biogenesis

Disease: Inosine Triphosphatase Deficiency