Western Blot (WB)
(Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit anti-Human, Mouse SPATA4 Polyclonal Antibody | anti-SPATA4 antibody
SPATA4 Antibody
Gene Names
SPATA4; SPEF1B; TSARG2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SPATA4 Antibody is affinity chromatography purified via peptide column.
Synonyms
SPATA4; Polyclonal Antibody; SPATA4 Antibody; SPEF1B; TSARG2; Spermatogenesis-associated protein 4; Testis and spermatogenesis cell-related protein 2; spermatogenesis associated 4; anti-SPATA4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
At least two isoforms of SPATA4 are known to exist; this antibody will detect both isoforms. SPATA4 antibody is predicted to not cross-react with SPATA protein family members
Purity/Purification
SPATA4 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
305
Applicable Applications for anti-SPATA4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SPATA4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA4.
Buffer
SPATA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SPATA4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of SPATA4 in mouse stomach tissue lysate with SPATA4 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SPATA4 in mouse testis tissue with SPATA4 antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of SPATA4 in mouse testis tissue with SPATA4 antibody at 20 μg/mL.)
Related Product Information for anti-SPATA4 antibody
SPATA4 Antibody: The spermatogenesis associated protein 4 (SPATA4) protein, also known as TSARG1, is highly expressed in testis, and to a much lower extent in other tissues. SPATA4 contains a highly conserved DUF1042 domain, but no significant homology with any other known protein. SPATA4 is thought to play an important role in the development of cryptorchid testis and is a testis-specific apoptosis candidate oncogene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,751 Da
NCBI Official Full Name
spermatogenesis-associated protein 4
NCBI Official Synonym Full Names
spermatogenesis associated 4
NCBI Official Symbol
SPATA4
NCBI Official Synonym Symbols
SPEF1B; TSARG2
NCBI Protein Information
spermatogenesis-associated protein 4; testis and spermatogenesis cell related protein 2; testis and spermatogenesis cell-related protein 2; testis spermatocyte apoptosis-related gene 2 protein
UniProt Protein Name
Spermatogenesis-associated protein 4
Protein Family
UniProt Gene Name
SPATA4
UniProt Synonym Gene Names
TSARG2
UniProt Entry Name
SPAT4_HUMAN
Uniprot Description
SPATA4:
Chromosomal Location of Human Ortholog: 4q34.2
Cellular Component: cytoplasm
Research Articles on SPATA4
Similar Products
Product Notes
The SPATA4 spata4 (Catalog #AAA150351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPATA4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPATA4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SPATA4 antibody can be used for detection of SPATA4 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SPATA4 spata4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPATA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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