Goat ITIH4 / gp120 Polyclonal Antibody | anti-ITIH4 antibody

Goat Polyclonal to Human ITIH4 / gp120

Cat. Num. AAA246939

• Gene Names: ITIH4; H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
• Reactivity: Human; Predicted Reactivity: Gorilla, Orangutan (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ITIH4 / gp120; Polyclonal Antibody; Goat Polyclonal to Human ITIH4 / gp120; Anti-ITIH4 / gp120 Antibody (C-Terminus) IHC-plus; ITIH4; gp120; H4P; ITIHL1; PK120; ITI heavy chain H4; PK-120; IHRP; ITI-HC4; Human ITIH4; anti-ITIH4 antibody

• Host: Goat
• Reactivity: Human; Predicted Reactivity: Gorilla, Orangutan (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ITIH4. This antibody is expected to recognize both reported isoforms (NP_002209.2; NP_001159921.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 930

• Applicable Applications for anti-ITIH4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (5 ug/ml), WB (0.03 - 0.1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 150kD band observed in Human Placenta lysates (calculated MW of 103kD according to NP_002209.2). The observed molecular weight corresponds to the glycosylated form. Recomm...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SDDGRRTLRVQGND from the C-terminus of human ITIH4 (NP_002209.2; NP_001159921.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (93%), Orangutan (93%), Gibbon (86%), Monkey (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: ITIH4 / gp120 antibody was raised against synthetic peptide C-SDDGRRTLRVQGND from the C-terminus of human ITIH4 (NP_002209.2; NP_001159921.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (93%), Orangutan (93%), Gibbon (86%), Monkey (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ITIH4 antibody IHC of human testis, Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(ITIH4 antibody (0.03 ug/ml) staining of Human Placenta lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 31542984
• NCBI GeneID: 3700
• NCBI Accession #: NP_002209.2
• NCBI GenBank Nucleotide #: NM_002218.4
• UniProt Accession #: Q14624; Q15135; Q9P190; Q9UQ54; B7Z545; E9PGN5
• Molecular Weight: 93,852 Da
• NCBI Official Full Name: inter-alpha-trypsin inhibitor heavy chain H4 isoform 1
• NCBI Official Synonym Full Names: inter-alpha-trypsin inhibitor heavy chain family, member 4
• NCBI Official Symbol: ITIH4
• NCBI Official Synonym Symbols: H4P; IHRP; GP120; PK120; ITIHL1; PK-120; ITI-HC4
• NCBI Protein Information: inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; inter-alpha-inhibitor heavy chain 4; plasma kallikrein-sensitive glycoprotein 120; inter-alpha-trypsin inhibitor, heavy chain-like, 1; inter-alpha-trypsin inhibitor family heavy chain-related protein; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein)
• UniProt Protein Name: Inter-alpha-trypsin inhibitor heavy chain H4
• Protein Family: Inter alpha-trypsin inhibitor
• UniProt Gene Name: ITIH4
• UniProt Synonym Gene Names: IHRP; ITIHL1; PK120; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; IHRP; Gp120; PK-120
• UniProt Entry Name: ITIH4_HUMAN

NCBI Description

The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

ITIH4: May be involved in acute phase reactions. Belongs to the ITIH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytoplasm; extracellular region; plasma membrane

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: response to cytokine stimulus; acute-phase response; hyaluronan metabolic process

Disease: Hypercholesterolemia, Familial