Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Epithelial Cell Transforming Sequence 2 (ECT2) Polyclonal Antibody | anti-ECT2 antibody
Polyclonal Antibody to Epithelial Cell Transforming Sequence 2 (ECT2)
MGHHHHHHSGSEF- EGQRGGPI LAPEEIKTIF GSIPDIFDVH TKIKDDLEDL IVNWDESKSI GDIFLKYSKD LVKTYPPFVN FFEMSKETII KCEKQKPRFH AFLKINQAKP ECGRQSLVEL LIRPVQRLPS VALLLNDLKK HTADENPDKS TLEKAIGSLK EVMTHINEDK RKTEAQKQIF DVVYEVDGCP ANLLSSHRSL VQRVETI
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(FITCstainingonIHC-P;Simple:A549cells.)
Immunohistochemistry (IHC)
(FITCstainingonIHC-P;Simple:A549cells.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Prostate cancer Tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]
Uniprot Description
ECT2: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)- induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3q26.31
Cellular Component: bicellular tight junction; cleavage furrow; cytoplasm; cytosol; intercellular junction; midbody; nucleus
Molecular Function: GTPase activator activity; guanyl-nucleotide exchange factor activity; protein binding; protein homodimerization activity; Rho guanyl-nucleotide exchange factor activity; signal transducer activity
Biological Process: activation of protein kinase activity; cell morphogenesis; cellular response to calcium ion; cellular response to hydrogen peroxide; cellular response to ionizing radiation; G-protein coupled receptor signaling pathway; mitotic cytokinesis; positive regulation of apoptosis; positive regulation of cytokinesis; positive regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of neuron differentiation; positive regulation of protein import into nucleus; protein homooligomerization; protein transport; regulation of attachment of spindle microtubules to kinetochore; regulation of protein kinase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction
Research Articles on ECT2
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Product Notes
The ECT2 ect2 (Catalog #AAA2006341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Epithelial Cell Transforming Sequence 2 (ECT2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Epithelial Cell Transforming Sequence 2 (ECT2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ECT2 ect2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- EGQRGGPI LAPEEIKTIF GSIPDIFDVH TKIKDDLEDL IVNWDESKSI GDIFLKYSKD LVKTYPPFVN FFEMSKETII KCEKQKPRFH AFLKINQAKP ECGRQSLVEL LIRPVQRLPS VALLLNDLKK HTADENPDKS TLEKAIGSLK EVMTHINEDK RKTEAQKQIF DVVYEVDGCP ANLLSSHRSL VQRVETI. It is sometimes possible for the material contained within the vial of "Epithelial Cell Transforming Sequence 2 (ECT2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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