Rabbit anti-Human, Mouse ITGB1BP3 Polyclonal Antibody | anti-ITGB1BP3 antibody

ITGB1BP3, ID (NMRK2, ITGB1BP3, NRK2, Nicotinamide riboside kinase 2, Integrin beta-1-binding protein 3, Muscle integrin-binding protein, Nicotinic acid riboside kinase 2, Ribosylnicotinamide kinase 2, Ribosylnicotinic acid kinase 2) (APC)

Cat. Num. AAA6311269

• Gene Names: NMRK2; MIBP; NRK2; ITGB1BP3
• Reactivity: Human, Mouse
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: ITGB1BP3; Polyclonal Antibody; ID (NMRK2; NRK2; Nicotinamide riboside kinase 2; Integrin beta-1-binding protein 3; Muscle integrin-binding protein; Nicotinic acid riboside kinase 2; Ribosylnicotinamide kinase 2; Ribosylnicotinic acid kinase 2) (APC); NMRK2; Ribosylnicotinic acid kinase 2; anti-ITGB1BP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ITGB1BP3. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ITGB1BP3 antibody: Western Blot (WB), Flow Cytometry (FC/FACS), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 156-186 in the Central region of human ITGB1BP3.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ITGB1BP3 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates ITGB1BP3 antibody detected ITGB1BP3 protein (arrow).)

Flow Cytometry (FC/FACS)

(ITGB1BP3 Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-ITGB1BP3 antibody

ITGB1BP3 catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). ITGB1BP3 reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. ITGB1BP3 may play a role in the regulation of terminal myogenesis.

Product Categories/Family for anti-ITGB1BP3 antibody

Antibodies; Protein Kinases

References

1. Bieganowski, P., et al. Cell 117(4):495-502(2004). 2. Li, J., et al. Dev. Biol. 261(1):209-219(2003). 3. Battle, M.A., et al. Biochemistry 42(24):7270-7282(2003). 4. Li, J., et al. J. Cell Biol. 147(7):1391-1398(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 24762248
• NCBI GeneID: 27231
• NCBI Accession #: NP_733778.1
• NCBI GenBank Nucleotide #: NM_170678.2
• UniProt Accession #: Q9NPI5; Q52M81; Q9NZK3; B7ZKR3
• Molecular Weight: 26046
• NCBI Official Full Name: nicotinamide riboside kinase 2 isoform 2
• NCBI Official Synonym Full Names: nicotinamide riboside kinase 2
• NCBI Official Symbol: NMRK2
• NCBI Official Synonym Symbols: MIBP; NRK2; ITGB1BP3
• NCBI Protein Information: nicotinamide riboside kinase 2
• UniProt Protein Name: Nicotinamide riboside kinase 2
• UniProt Gene Name: NMRK2
• UniProt Synonym Gene Names: ITGB1BP3; NRK2; NRK 2; NmR-K 2; MIBP; RNK 2
• UniProt Entry Name: NRK2_HUMAN

Uniprot Description

ITGB1BP3: an enzyme that catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside to form nicotinamide mononucleotide and nicotinic acid mononucleotide. Reduces laminin matrix deposition and cell adhesion to laminin, but not to fibronectin. Interacts with ITGB1 alone or when associated with alpha-7, but not with alpha-5. Involved in the regulation of PXN at the protein level and of PXN tyrosine phosphorylation. Predominantly expressed in skeletal muscle and, at a much lower level, in the heart (at protein level). No expression in brain, kidney, liver, lung, pancreas nor placenta. May play a role in the regulation of terminal myogenesis. Two alternatively spliced human isoforms have been described.

Protein type: EC 2.7.1.22; Cell adhesion; EC 2.7.1.173; Cell development/differentiation; Kinase, other

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: intracellular

Molecular Function: protein binding; ribosylnicotinamide kinase activity; metal ion binding; ATP binding

Biological Process: NAD biosynthetic process; negative regulation of myoblast differentiation; phosphorylation